As far as I know the vast majority of commercial culture media possess components which are very similar. The main concern I have today is the lack of consensus as regards of single or sequential media, and the difference in oxygen in incubators. In Brazil, we live a stage in which all embryologists are measuring pH of the medium inside the incubator without a predefined protocol. I would appreciate if someone could tell me if they have a specific protocol to be used in the incubator to measure medium pH, and if Marcos could clarify what exactaly he meant by "incorrect set up in culture media in the lab". Gilberto Almodin, MD, PhD.
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