Pinopodes, Leukemia Inhibitory Factor, Integrin-β3, and Mucin-1 Expression in the Peri-implantation Endometrium of Women with Unexplained Recurrent Pregnancy Loss

Mucin-1 expression is lower in peri-implantation endometrium of women with recurrent pregnancy loss than in controls, but pinopode morphology and coverage, and leukemia inhibitory factor and integrin-b3 expression are similar.

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Authors

Bufang Xu, Ph.D., Xiaoxi Sun, Ph.D., Lu Li, M.S., Ling Wu, M.S., Aijun Zhang, Ph.D., Yun Feng, M.D., Ph.D.

Vol 98, Issue 2 , Pages 389-395

Abstract

Objective:

To compare pinopode morphology and coverage, and expression of leukemia inhibitory factor (LIF), integrin-β3, and mucin-1 (MUC1) in the peri-implantation endometrium of women with unexplained recurrent pregnancy loss (RPL) versus normal fertile women.

Design:

Exploratory clinical study.

Setting:

University-affiliated assisted reproductive center.

Patient(s):

Women undergoing assisted reproduction at centers of reproductive medicine in two university teaching hospitals.

Intervention(s):

Endometrial biopsy samples were obtained from women with RPL (test group, n = 30) and normal fertile women (control group, n = 26) 7 days after the day of luteinizing hormone peak. Pinopode morphology and coverage were assessed by use of scanning electron microscopy. Expression of LIF, integrin β3, and MUC1 were evaluated by immunohistochemical staining.

Main Outcome Measure(s):

Pinopode morphology and coverage, and the expression of LIF, integrin-β3, and MUC1 in both groups.

Result(s):

Pinopode morphology and coverage, and the expression of LIF and integrin-β3 showed no statistically significant differences between the two groups. The expression of MUC1 was statistically significantly reduced in the glandular epithelial cells and endometrial lumen of the RPL group compared with controls.

Conclusion(s):

The morphology and the coverage of pinopodes and expression of LIF and integrin-β3 did not change, but MUC1 expression was statistically significantly reduced in women with RPL compared with normal women.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)00487-6/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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