Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in PVP-based sperm immobilization medium

Polyvinylpyrrolidone-based medium can induce sperm DNA fragmentation during intracytoplasmic sperm injection, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.

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Authors

Sujit Raj Salian, B.Sc., Guruprasad Kalthur, Ph.D., Shubhashree Uppangala, M.Sc., Pratap Kumar, M.D., Satish Kumar Adiga, Ph.D.

Vol 98, Issue 2, Pages 321-325

Abstract

Objective:

To elucidate the effect of sperm immobilization media that are and are not based on polyvinylpyrrolidone (PVP) on the DNA integrity of fresh and frozen-thawed spermatozoa during standard intracytoplasmic sperm injection (ICSI) conditions.

Design:

Experimental prospective study.

Setting:

Embryology research laboratory.

Patient(s):

Forty-six ejaculates from normozoospermic and oligozoospermic men.

Intervention(s):

Assessment of sperm DNA fragmentation by single-cell gel electrophoresis assay.

Main Outcome Measure(s):

DNA integrity of fresh and frozen-thawed spermatozoa from normozoospermic and oligozoospermic ejaculates exposed to PVP-based and non-PVP-based media.

Result(s):

Exposure of fresh and frozen thawed spermatozoa from normozoospermic and oligozoospermic ejaculates to PVP-based medium in an ICSI dish for 30 minutes statistically significantly increased the DNA fragmentation. In contrast, the extent of DNA fragmentation in non-PVP-based medium did not statistically significantly differ from control.

Conclusion(s):

A PVP-based medium can induce a statistically significant amount of sperm DNA fragmentation in an ICSI dish, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)00495-5/fulltext


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