Sirt1 exerts anti-inflammatory effects and promotes steroidogenesis in Leydig cells

Sirt1 plays protective roles and promotes steroidogenesis in Leydig cells through its anti-inflammatory actions and coactivation of SF-1.

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Authors

Ling Wu, M.Sc., Aijun Zhang, Ph.D., Yijuan Sun, Ph.D., Xiaobin Zhu, M.Sc., Weimin Fan, M.Sc., Xiaowei Lu, M.Sc., Qingqing Yang, M.Sc., Yun Feng, M.D., Ph.D.

Vol 98, Issue 1 , Pages 194-199

Abstract

Objective:

To investigate the anti-inflammatory effects of Sirt1 and its roles in steroidogenesis in Leydig cells.

Design:

In vitro cell culture study.

Setting:

Reproductive medical center.

Animal(s):

C57BL/6 male mice.

Intervention(s):

TM3 Leydig cells were treated with proinflammatory cytokines including tumor necrosis factor-α, interleukin-6, or interleukin-1β.

Main Outcomes Measure(s):

Sirt1 mRNA and protein levels were measured by real-time polymerase chain reaction and Western blotting in TM3 Leydig cells treated with proinflammatory cytokines. The cell viability was determined using MTT and BrdU incorporation assays. The effect of Sirt1 on the coactivation of steroidogenic factor 1 (SF-1) was characterized by coimmunoprecipitation and luciferase reporter assays.

Result(s):

Sirt1 mRNA and protein levels were significantly down-regulated by proinflammatory cytokines treatment in TM3 Leydig cells. Sirt1 agonist or overexpression could efficiently protect cytotoxicity induced by proinflammatory cytokines. Sirt1 promoted steroidogenesis through its coactivation of SF-1.

Conclusion(s):

Sirt1 plays protective roles and promotes steroidogenesis in Leydig cells through its anti-inflammatory actions and coactivation of SF-1.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)00439-6/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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