Studying nonobstructive azoospermia in cystinosis: histologic examination of testes and epididymis and sperm analysis in a Ctns−/− mouse model

Male cystinosis patients are infertile. Male Ctns-/- mice (cystinosis mouse model) showed normal fertility compared with wild-type mice (measured by litter size). Also, histology of testes and epididymis as well as sperm analysis were similar in both groups.

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Authors

Martine T.P. Besouw, M.D., Ans M.M. van Pelt, Ph.D., Héloïse P. Gaide Chevronnay, Ph.D., Pierre J. Courtoy, M.D., Ph.D., Anna Pastore, Ph.D., Ellen Goossens, Ph.D., Olivier Devuyst, M.D., Ph.D., Corinne Antignac, M.D., Ph.D., Elena N. Levtchenko, M.D., Ph.D.

Vol 98, Issue 1 , Pages 162-165

Abstract

Objective:

To study the pathogenesis of male infertility in cystinosis due to nonobstructive azoospermia, using a Ctns−/− mouse model.

Design:

Observational case-control study.

Setting:

Academic research laboratory.

Animal(s):

Male C57BL/6 Ctns−/− mice were compared with C57BL/6 wild-type (wt) mice.

Intervention(s):

None.

Main Outcome Measure(s):

Fertility was studied using litter size (n = 3 vs. n = 2). After animals were sacrificed, testes, epididymis, and vas deferens were removed for testicular cystine measurements (n = 5 vs. n = 6), histologic studies (n = 3 vs. n = 3), and sperm analysis (n = 3 vs. n = 3).

Result(s):

Mean testicular cystine content was significantly higher in Ctns−/− mice compared with wt mice (26.6 ± 1.22 vs. 0.1 ± 0.01 nmol cystine/mg protein). Testes of Ctns−/− mice had lower weight compared with wt mice (0.096 ± 0.009 g vs. 0.112 ± 0.004 g), but mice fertility was similar (litter size 6.6 ± 1.4 vs. 6.3 ± 2.6 pups). Neither histologic nor sperm abnormalities were found.

Conclusion(s):

The Ctns−/− mouse model generated on C57BL/6 background is not suitable for clarifying the pathogenesis of male infertility in cystinosis. The etiology of nonobstructive azoospermia in these patients remains unclear.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)00388-3/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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