Comparison of endometrial prostanoid profiles in three infertile subgroups: the missing part of receptivity?
Article In Press
Irem Demiral Keleş, M.D., Ege Ülgen, M.D., Melike Belkız Erkan, B.Sc., M.S., Ph.D., Saliha Esin Çelik, Ph.D., Yasemin Aydın, Ph.D., Ayşe Nur Önem, M.Sc., Hülya Kandemir, M.D., Tuğçe Arslanoğlu, M.D., Mustafa Reşat Apak, Ph.D., Uğur Sezerman, Ph.D., John Yeh, M.D., Faruk Buyru, M.D., Ercan Baştu, M.D.
To study the prostanoid profile of the endometria of patients with recurrent implantation failure (RIF), unexplained infertility (UIF), and recurrent miscarriages (RM), and to compare them with the endometria of healthy fertile controls.
Prospective cohort study.
Fifteen patients with RIF, 18 patients with UIF, 16 patients with RM, and 23 fertile controls were recruited.
Endometrial samples were taken during the window of implantation. After tissue homogenization and extraction, analysis with ultra-performance liquid chromatography diode array detector electrospray ionisation tandem mass spectrometry was performed.
Main Outcome Measures
Concentrations of prostaglandin (PG) D1, PGE1, PGF1α, 6-ketoPGF1α, PGD2, PGE2, PGF2α, 15-deoxy-Δ12,14-PGJ2, PGD3, PGE3, PGF3α, thromboxane B2, 13,14-dihydro-PGE1, 13,14-dihydro-PGF1α, 13,14-dihydro-PGF2α, 13,14-dihydro-15-keto-PGE1, 13,14-dihydro-15-keto-PGE2, and 13,14-dihydro-15-keto-PGF2α were assessed.
Comparison of the endometria of patients with UIF and the controls showed no statistically significant differences. When the endometria of patients with RIF were compared with the controls, thromboxane B2 (TXB2) was found significantly higher (843.1 pg/mg vs. 133.5 pg/mg). When the endometria of patients with RM were compared with controls, 13,14-dihydro-15-keto PGF2α and TXB2 were found significantly higher (3907.30 pg/mg vs. 17.80 pg/mg and 858.7 pg/mg vs. 133.5 pg/mg respectively).
We identified increased endometrial presence of TXB2 in patients with RM and RIF, and 13,14-dihydro-15-keto PGF2α in patients with RM. Although common ground is observed for RM and RIF, prostanoids, on the other hand, might make their own contribution to endometrial receptivity as important as genes and proteins. Attempts to normalize the prostaglandin profile of the endometrium via enzymatic activity can open new therapeutic options.