Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation

After ejaculation, spermatozoa are still able to take up exosomes, but only exosomes from normozoospermic individuals improve spermatozoa motility and trigger capacitation, while those from asthenozoospermic men fail to exert any positive function.

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Volume 111, Issue 5, Pages 897–908.e2

Authors:

Valentina Murdica, Ph.D., Elisa Giacomini, Ph.D., Alessandra Alteri, Ph.D., Alessandro Bartolacci, M.Sc., Greta Chiara Cermisoni, M.Sc., Natasa Zarovni, Ph.D., Enrico Papaleo, M.D., Francesco Montorsi, M.D., Andrea Salonia, M.D., Paola Viganò, Ph.D., Riccardo Vago, Ph.D.

Abstract:

Objective

To characterize in depth and investigate the role of exosomes present in seminal plasma in affecting parameters underlying sperm activity.

Design

In vitro experimental study.

Setting

Research hospital.

Patient(s)

Normozoospermic, severe asthenozoospermic, and post-vasectomy azoospermic men 18–55 years of age were considered for the study. Seminal plasma was collected and processed to separate spermatozoa and exosomes.

Intervention(s)

None.

Main Outcomes Measure(s)

Exosomes from seminal plasma were isolated and characterized by means of nanoparticle tracking analysis, transmission electron microscopy and Western blot. Exosome uptake by spermatozoa was monitored by means of immunofluorescence and flow cytometry. The effect of exosomes on spermatozoa was determined by evaluating progressive motility and capacitation, the latter assessed by means of tyrosine phosphorylation and acrosome reaction.

Result(s)

We isolated and characterized exosomes from seminal plasma of normo-, astheno-, and azoospermic patients. They display similar features in terms of shape, size, expression of canonic exosome markers and proteins involved in spermatozoa maturation, and fertilization capacity. After ejaculation, sperm cells are still receptive and are able to take up exosomes in a time- and pH-dependent manner. Exosomes derived from normozoospermic but not from asthenozoospermic individuals improve spermatozoa motility and trigger capacitation. Transfer of cysteine-rich secretory protein 1 from exosomes to spermatozoa may have a role in these phenomena.

Conclusion(s)

These findings provide evidence that: 1) sperm can still receive vesicle-derived cargo after ejaculation; 2) sperm motility and ability to undergo capacitation can benefit from exosomal transfer; and 3) semen quality is affected by male tract exosomes.


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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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