Extracellular matrix signaling activates differentiation of adult ovary-derived oogonial stem cells in a species-specific manner

Article In Press

0
0

Authors:

Julie A. MacDonald, B.S.a, Yasushi Takai, M.D., Ph.D.b, Osamu Ishihara, M.D., Ph.D.b, Hiroyuki Seki, M.D., Ph.D.b, Dori C. Woods, Ph.D.a, Jonathan L. Tilly, Ph.D.

Abstract:

Objective

To test if ovarian microenvironmental cues affect oogonial stem cell (OSC) function in a species-specific manner.

Design

Animal and human study.

Setting

Research laboratory.

Patient(s)/Animal(s)

Human ovarian cells obtained from cryopreserved ovarian cortical tissue of reproductive-age women, and ovarian cells and tissues from female C57BL/6 mice.

Intervention(s)

Mouse ovarian tissue, mouse OSCs (mOSCs) and human OSCs (hOSCs) were analyzed for extracellular matrix (ECM) protein expression, and OSCs isolated from adult mouse and human ovaries were cultured in the absence or presence of ECM proteins without or with an integrin signaling inhibitor.

Main Outcome Measure(s)

Gene expression and in vitro derived (IVD) oocyte formation.

Result(s)

Culture of mOSCs on a collagen-based ECM significantly elevated the rate of differentiation of the cells into IVD oocytes. Mouse OSCs expressed many integrins, including Arg-Gly-Asp (RGD)–binding subunits, and ECM-mediated increases in mOSC differentiation were blocked by addition of integrin-antagonizing RGD peptides. In comparison, hOSCs expressed a different pattern of integrin subunits compared with mOSCs, and hOSCs were unresponsive to a collagen-based ECM; however, hOSCs exhibited increased differentiation into IVD oocytes when cultured on laminin.

Conclusion(s)

These data, along with in silico analysis of ECM protein profiles in human ovaries, indicate that ovarian ECM-based niche components function in a species-specific manner to control OSC differentiation.


Read the full text here.

Go to the profile of Fertility and Sterility

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

No comments yet.