Ninety babies born after round spermatid injection into oocytes: Survey of their development from fertilization up to 2 years old.

This 2-year follow-up study of round spermatid injection babies for physical and cognitive ability show no difference from naturally conceived babies.

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Volume 110, Issue 3, Pages 443–451

Authors:

Atsushi Tanaka, M.D., Ph.D., Kohta Suzuki, M.D., Ph.D., M.P.H., Motoi Nagayoshi, M.D., Akihiro Tanaka, M.B.I.T., Youichi Takemoto, Seiji Watanabe, Ph.D., Satoru Takeda, M.D., Ph.D., Minoru Irahara, M.D., Ph.D., Naoaki Kuji, M.D., Ph.D., Zentaro Yamagata, M.D., Ph.D., Ryuzo Yanagimachi, Ph.D.

Abstract:

Objective

To compare physical and cognitive development of babies born after round spermatid injection (ROSI) with those born after natural conception.

Design

Comparison of efficiencies of ROSI and ICSI using testicular spermatozoa, performed in the St. Mother Clinic. Physical and cognitive development of ROSI babies recorded by parents in the government-issued Mother-Child Handbook was checked and verified by attending pediatricians. Data included baby's weight gain and response to parents' voice/gesture.

Setting

Assisted reproduction technology practice.

Patient(s)

A total of 721 men participated in ROSI; 90 ROSI babies were followed for 2 years for their physical and cognitive development. Control subjects were 1,818 naturally born babies.

Intervention(s)

Surgical retrieval of spermatogenic cells from testes; selection and injection of round spermatids into oocytes; oocyte activation, in vitro culture of fertilized eggs, and embryo transfer to mothers.

Main Outcome Measure(s)

Physical and cognitive development of ROSI babies (e.g., body weight increase, response to parents, and understanding and speaking simple language) compared with naturally born babies.

Result(s)

Of 90 ROSI babies, three had congenital aberrations at birth, which corrected spontaneously (ventricular septa) or after surgery (cleft lip and omphalocele). Physical and cognitive development of ROSI babies was similar to those of naturally born babies.

Conclusion(s)

There were no significant differences between ROSI and naturally conceived babies in either physical or cognitive development during the first 2 years after birth.

Clinical Trial Registration Number

UMIN Clinical Trials Registry UMIN000006117.


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2 Comments

Go to the profile of Jan Tesarik
Jan Tesarik 3 months ago

Healthy babies born after round spermatid injection into human oocytes

 

Jan Tesarik, Carmen Mendoza, Raquel Mendoza-Tesarik

 

Molecular Assisted Reproduction and Genetics (MARGen) Clinic, Camino de Ronda 2, 18006 Granada, Spain

 

In the latest issue of Fertility and Sterility Tanaka et al. report a survey of the development of ninety babies conceived by round spermatid injection (ROSI) from fertilization to 2 years of age (1). They did not find any significant differences between these ROSI babies as compared with naturally conceived babies. We would like to congratúlate the authors for these excellent results. More than twenty years ago, in 1995, we reported the birth of the world´s first two babies spermatid by ROSI (2). Since then, ROSI was used at our clinic in 118 cases between September 2005 and December 2014.  Fertilization was achieved in 46 (39%) cases, and in 42 of them at least one cleaving embryo was available for transfer to the uterus. Eighteen patients (15% of the started ROSI treatment attempts) became pregnant after embryo transfer, and 15 pregnancies resulted in childbirth. Seventeen babies were born, all of which were normal at birth and are developing normally so far. As of today, none of these babies, some of whom are approaching the puberty, show any evidence of epigenetic disorders, such as Prader-Willi syndrome, Angelman syndrome or Wiskott-Aldrich syndrome, or any other kind of physical or mental aberration. The interpretation of these data are in agreement with the conclusion of Tanaka et al. (1) in that ROSI can be considered a clinically useful option for cases of spermatogenesis arrest at the round spermatid stage.

 

Other  ROSI babies are likely to be born at different clinics worldwide.The fact that no ROSI-related abnormalities were detected is encouraging, even though the number of cases is still low. We suggest that an international databank on ROSI obstetrical and pediatric outcomes should be created, and all clinics having achieved childbirths should be invited to share their experience with the aim to further improve the safety of this technology.

 

References

 

1. Tanaka A, Suzuki K, Nagayoshi M, Tanaka A, Takemoto Y, Watanabe S, Takeda S, Irahara M, Kuji N, Yamagata Z, Yanagimachi R (2018) Ninety babies born after round spermatid injection into oocytes: survey of their development from fertilization to 2 years of age. Fertil Steril 110(3): 443-450.

2 Tesarik J, Mendoza C, Testart J (1995) Viable embryos from Injection of round spermatids into oocytes. N Engl J Med 333:525. 

Go to the profile of Jan Tesarik
Jan Tesarik 3 months ago

Healthy babies born after round spermatid injection into human oocytes

 

Jan Tesarik, Carmen Mendoza, Raquel Mendoza-Tesarik

 

Molecular Assisted Reproduction and Genetics (MARGen) Clinic, Camino de Ronda 2, 18006 Granada, Spain

 

In the latest issue of Fertility and Sterility Tanaka et al. report a survey of the development of ninety babies conceived by round spermatid injection (ROSI) from fertilization to 2 years of age (1). They did not find any significant differences between these ROSI babies as compared with naturally conceived babies. We would like to congratúlate the authors for these excellent results. More than twenty years ago, in 1995, we reported the birth of the world´s first two babies spermatid by ROSI (2). Since then, ROSI was used at our clinic in 118 cases between September 2005 and December 2014.  Fertilization was achieved in 46 (39%) cases, and in 42 of them at least one cleaving embryo was available for transfer to the uterus. Eighteen patients (15% of the started ROSI treatment attempts) became pregnant after embryo transfer, and 15 pregnancies resulted in childbirth. Seventeen babies were born, all of which were normal at birth and are developing normally so far. As of today, none of these babies, some of whom are approaching the puberty, show any evidence of epigenetic disorders, such as Prader-Willi syndrome, Angelman syndrome or Wiskott-Aldrich syndrome, or any other kind of physical or mental aberration. The interpretation of these data are in agreement with the conclusion of Tanaka et al. (1) in that ROSI can be considered a clinically useful option for cases of spermatogenesis arrest at the round spermatid stage.

 

Other  ROSI babies are likely to be born at different clinics worldwide.The fact that no ROSI-related abnormalities were detected is encouraging, even though the number of cases is still low. We suggest that an international databank on ROSI obstetrical and pediatric outcomes should be created, and all clinics having achieved childbirths should be invited to share their experience with the aim to further improve the safety of this technology.

 

References

 

1. Tanaka A, Suzuki K, Nagayoshi M, Tanaka A, Takemoto Y, Watanabe S, Takeda S, Irahara M, Kuji N, Yamagata Z, Yanagimachi R (2018) Ninety babies born after round spermatid injection into oocytes: survey of their development from fertilization to 2 years of age. Fertil Steril 110(3): 443-450.

2 Tesarik J, Mendoza C, Testart J (1995) Viable embryos from Injection of round spermatids into oocytes. N Engl J Med 333:525.