Skewed X-chromosome inactivation and shorter telomeres associate with idiopathic premature ovarian insufficiency

Shortened telomere length and skewed XCI are associated with idiopathic premature ovarian insufficiency that does not present alterations in the X-linked genes AR and FMR1.

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Volume 110, Issue 3, Pages 476–485.e1

Authors:

Cristiana L. Miranda-Furtado, Ph.D., Heloise R. Luchiari, M.Sc., Daiana C. Chielli Pedroso, M.Sc., Gislaine S. Kogure, Ph.D., Lisandra C. Caetano, Ph.D., Bárbara A. Santana, Ph.D., Viviane P. Santana, M.Sc., Cristina L. Benetti-Pinto, M.D., Ph.D., Fernando M. Reis, M.D., Ph.D., Mariella A. Maciel, M.D., Rui A. Ferriani, M.D., Ph.D., Ester S. Ramos, M.D., Ph.D., Rodrigo T. Calado, M.D., Ph.D., Rosana M. dos Reis, M.D., Ph.D.

Abstract:

Objective

To analyze whether telomere length, X-chromosome inactivation (XCI), and androgen receptor (AR) GAG polymorphism are related to idiopathic premature ovarian insufficiency (POI).

Design

Case–control study.

Setting

University hospital.

Patient(s)

A total of 121 women, including 46 nonsyndromic POI and 75 controls.

Intervention(s)

None.

Main Outcome Measure(s)

Age, weight, height, body mass index (BMI), systolic and diastolic arterial pressure, E2, androstenedione, T, and C-reactive protein were assessed. Telomere length was estimated by quantitative real-time polymerase chain reaction, XCI was measured using the Human Androgen Receptor and X-linked retinitis pigmentosa 2 (RP2) methylation assays. AR and FMR1 polymorphism was assessed by quantitative fluorescent polymerase chain reaction and sequencing.

Result(s)

Premature ovarian insufficiency women had a higher mean age, weighed less, and exhibited lower C-reactive protein, E2, and androstenedione levels. The AR polymorphism did not differ between the groups. Four patients had premutation (55–200 CGG repeats), and none displayed a full mutation in the FMR1 gene. However, patients with POI showed shorter telomere length and higher frequency of skewed XCI. Extreme skewing (≥90%) was observed in 15% of women with POI, and shorter telomeres correlated with XCI skewing in both groups.

Conclusion(s)

Skewed XCI and shortened telomere length were associated with idiopathic POI, despite no alterations in the AR and FMR1 genes. Additionally, there is a tendency for women with short telomeres to exhibit skewed XCI.


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Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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