Kinetics of meiotic maturation in oocytes from unstimulated ovaries and duration of pronucleus presence and preimplantation development

Gamete Biology

VOLUME 1, ISSUE 2, P124-131, NOVEMBER 01, 2020


Hiromitsu Shirasawa, M.D., Ph.D., Yukiyo Kumazawa, M.D., Ph.D., Kazumasa Takahashi, Ph.D., Mayumi Goto, A.A, Wataru Sato, M.D., Ph.D., Natsuki Ono, M.D., Ph.D., Kazue Togashi, M.D., Ph.D., Kenichi Makino, M.D., Ph.D., Masato Waga, M.D., Ph.D., Naoki Sato, M.D., Ph.D., Yukihiro Terada, M.D., Ph.D.



To evaluate the meaning of meiotic maturation kinetics and duration of pronucleus presence (DPP) for parthenogenetic activation outcome.


Retrospective study.


University hospital.


Eight patients with endometrioid adenocarcinoma and 65 patients who underwent in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI).


After collection of oocytes from nonstimulated ovaries of patients with endometrioid adenocarcinoma, in vitro maturation (IVM) and parthenogenetic activation performed with time-lapse imaging; after ICSI, embryos similarly incubated with time-lapse imaging.

Main Outcome Measure(s)

Timing of the release of the first polar body (fPB), DPP, and developmental stage with IVM and parthenogenetic activation; after ICSI, assessment of DPP and preimplantation developmental stage.


With IVM, 55.2% of oocytes matured; 53.1% of fPBs were released within 24 hours, and 46.9% of fPBs were released after 24 hours. Regarding developmental stage, oocytes that released fPB later during IVM tended to develop more than oocytes that released the fPB within 24 hours. For embryos from parthenogenetic activation the DPP was statistically significantly shorter than the DPP of embryos from ICSI. With ICSI, the DPP was statistically significantly shorter in embryos that developed to ≥8 cells than embryos whose final development included ≤7 cells. The development rate in parthenogenetic activation was statistically significantly lower than that in ICSI.


Embryo development is negatively affected by DPP that is too short or too long. When the DPP was short with parthenogenetic activation, embryo development did not proceed, indicating that DPP is an important determinant of parthenogenetic activation outcomes as with the timing of fPB release.

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