Telomere aberrations, including telomere loss, doublets, and extreme shortening, are increased in patients with infertility

We demonstrate that telomere aberrations represent a major common characteristic of patients with infertility and that telomere loss is related to chromosomal aberrations. We propose telomere aberrations to be used as a diagnostic marker of infertility

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VOLUME 115, ISSUE 1, P164-173

Authors:

Radhia M’kacher, Ph.D., Bruno Colicchio, Ph.D., Valentine Marquet, Ph.D., M.D., Claire Borie, Ph.D., M.D., Wala Najar, M.Sc., William M. Hempel, Ph.D., Leonhard Heidingsfelder, M.Sc., Noufissa Oudrhiri, Ph.D., Mustafa Al Jawhari, Ph.D., Nadège Wilhelm-Murer, M.Sc., Marguerite Miguet, Ph.D., M.D., Alain Dieterlen, Ph.D., Georges Deschênes, Ph.D., M.D., Anne-Claude Tabet, Ph.D., M.D., Steffen Junker, D.Phil., Michael Grynberg, Ph.D., M.D., Michael Fenech, Ph.D., Annelise Bennaceur-Griscelli, Ph.D., M.D., Philippe Voisin, Sc.D., Patrice Carde, M.D., Eric Jeandidier, M.D., Catherine Yardin, Ph.D., M.D.

Abstract:

Objective

To test the hypothesis that telomere shortening and/or loss are risk factors for infertility.


Design

Retrospective analysis of the telomere status in patients with infertility using conventional cytogenetic data collected prospectively.


Setting

Academic centers.


Patient(s)

Cytogenetic slides with cultured peripheral lymphocytes from 50 patients undergoing fertility treatment and 150 healthy donors, including 100 donors matched for age.


Intervention(s)

Cytogenetic slides were used to detect chromosomal and telomere aberrations.


Main Outcome Measure(s)

Telomere length and telomere aberrations were analyzed after telomere and centromere staining.


Result(s)

The mean telomere length of patients consulting for infertility was significantly less than that of healthy donors of similar age. Moreover, patients with infertility showed significantly more extreme telomere loss and telomere doublet formation than healthy controls. Telomere shortening and/or telomere aberrations were more pronounced in patients with structural chromosomal aberrations. Dicentric chromosomes were identified in 6/13 patients, with constitutional chromosomal aberrations leading to chromosomal instability that correlated with chromosomal end-to-end fusions.


Conclusion(s)

Our findings demonstrate the feasibility of analyzing telomere aberrations in addition to chromosomal aberrations, using cytogenetic slides. Telomere attrition and/or dysfunction represent the main common cytogenetic characteristic of patients with infertility, leading to potential implications for fertility assessment. Pending further studies, these techniques that correlate the outcome of assisted reproduction and telomere integrity status may represent a novel and useful diagnostic and/or prognostic tool for medical care in this field.

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.