Novel nonclassic progesterone receptor PGRMC1 pulldown-precipitated proteins reveal a key role during human decidualization

In nondecidualized and decidualized endometrial stromal cells, GST-PGRMC1 precipitates multiple proteins mainly implicated in adenosine triphosphate generation/transport activity, protein biosynthesis/posttranslational processing, and protection against oxidative stress.

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Authors:

Stefania Salsano, Ph.D., Roberto González-Martín, Ph.D., Alicia Quiñonero, Ms.C., Soraya López-Martín, Ms.C., Ana Pilar Gómez-Escribano, Ph.D., Silvia Pérez-Debén, Ph.D., Maria Yañez-Mo, Ph.D., Francisco Domínguez, Ph.D.

Abstract:

Objective

To investigate PGRMC1-precipitating proteins in human endometrial stromal cells (ESC) to understand its role during in vitro decidualization.

Design

Prospective observational study.

Setting

Academic fertility center.

Patient(s)

Fifteen fertile oocyte donors.

Intervention(s)

Isolated ESCs decidualized in vitro and used in pulldown assays.

Main Outcome Measure(s)

GST-PGRMC1-precipitated proteins identified in nondecidualized ESC (ndESC) and ESC decidualized via a long (8 days) or short (4 days) decidualization protocol (dESC).

Result(s)

Using pulldown assays and mass spectrometry, decidualization was evaluated by prolactin secretion (ELISA) and cytoskeleton morphology (F-actin staining). The protein interactions were validated by colocalization and coimmunoprecipitation. The pulldown and mass spectrometry analysis identified 21, 24, and 24 new significant GST-PGRMC1-precipitated proteins in ndESC, long dESC, and short dESC, respectively, compared with controls. The functional annotation analysis categorized these proteins mainly into endomembrane system and mitochondria cellular components, both related to adenosine triphosphate (ATP) generation and transport activity, protein biosynthesis and posttranslational processing, vesicle trafficking, and protection against oxidative stress activities. Monoamine oxidase B (MAOB) and B-cell receptor-associated protein 31 (BAP31) were identified in dESC from both decidualization protocols. PGRMC1–MAOB/BAP31 interactions were confirmed by immunofluorescence and coimmunoprecipitation in dESC.

Conclusion(s)

Novel GST-PGRMC1-precipitated proteins discovered in ESC suggest that this protein is implicated in deep remodeling of ESC during decidualization and aggregates mainly with proteins involved in biosynthesis, intracellular transport, and mitochondrial activity.



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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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