Conception by fertility treatment and offspring deoxyribonucleic acid methylation

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Authors:

Edwina H. Yeung, Ph.D., Pauline Mendola, Ph.D., Rajeshwari Sundaram, Ph.D., Xuehuo Zeng, Ph.D., Weihua Guan, Ph.D., Michael Y. Tsai, Ph.D., Sonia L. Robinson, Ph.D., Judy E. Stern, Ph.D., Akhgar Ghassabian, M.D., Ph.D., David Lawrence, Ph.D., Thomas G. O’Connor, Ph.D., James Segars, M.D., Robert E. Gore-Langton, Ph.D., Erin M. Bell, Ph.D.

Abstract:

Objective

To investigate whether deoxyribonucleic acid (DNA) methylation at birth and in childhood differ by conception using assisted reproductive technologies (ART) or ovulation induction compared with those in children conceived without fertility treatment.


Design

Upstate KIDS is a matched exposure cohort which oversampled on newborns conceived by treatment.


Setting

New York State (excluding New York City).


Patient(s)

This analysis included 855 newborns and 152 children at approximately 9 years of age.


Intervention(s)

None.


Main Outcome Measure(s)

DNA methylation levels were measured using the Illumina EPIC platform. Single CpG and regional analyses at imprinting genes were conducted.


Result(s)

Compared to no fertility treatment, ART was associated with lower mean DNA methylation levels at birth in 11 CpGs (located in/near SYCE1, SPRN, KIAA2013, MYO1D, GET1/WRB-SH4BGR, IGF1R, SORD, NECAB3/ACTL10, and GET1) and higher mean methylation level in 1 CpG (KLK4; all false discovery rate P<.05). The strongest association (cg17676129) was located at SYCE1, which codes for a synaptonemal complex that plays a role in meiosis and therefore infertility. This CpG remained associated with newborn hypomethylation when the analysis was limited to those conceived with ICSI, but this may be because of underlying male infertility. In addition, nine regions in maternally imprinted genes (IGF1R, PPIEL, SVOPL GNAS, L3MBTL, BLCAP, HYMAI/PLAGL1, SNU13, and MEST) were observed to have decreased mean DNA methylation levels among newborns conceived by ART. In childhood, hypomethylation of the maternally imprinted gene, GNAS, persisted. No CpGs or regions were associated with ovulation induction.


Conclusion(s)

ART but not ovulation induction was associated with hypomethylation at birth, but only one difference at an imprinting region appeared to persist in childhood.

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.