Coenzyme Q dependent mitochondrial respiratory chain activity in granulosa cells is reduced with aging
Coenzyme Q (CoQ)-dependent mitochondrial activity in granulosa cells reduces with aging and is restored via supplementation, indicating that functional CoQ10 deficit is the main underlying cause for oxidative phosphorylation dysfunction.
Assaf Ben-Meir, M.D., Shlomi Yahalomi, M.D., Brit Moshe, B.Sc., Yoel Shufaro, M.D., Ph.D., Benjamin Reubinoff, M.D., Ph.D., Ann Saada, Ph.D.
Volume 104, Issue 3, Pages 724-727
To examine coenzyme Q10 (CoQ10)-dependent mitochondrial respiratory chain (MRC) activity in granulosa cells (GC) with aging and examine the effect of in vitro CoQ supplementation.
Ten younger (<32 years) and 10 older (>39 years) patients undergoing in vitro fertilization (IVF) treatment.
Measurement of succinate-cytochrome c reductase (MRC complex II + III) activity in the presence and absence of CoQ1 (a soluble CoQ analog).
Main Outcome Measure(s):
MRC enzymatic activity in human GC via complex II + III measured in GC homogenate by spectrophotometry and compared with CoQ in dependent MRC complex II and citrate synthase (CS).
Complex II + III activity was 1.9 times higher in young patients compared with older patients (18.3 ± 5.8 and 9.6 ± 3 nmol/min/mg, respectively) whereas II and CS were not statistically significantly different. Increased II + III activity in the presence CoQ1 was observed in both groups but was statistically significantly higher in the older patients, reaching similar levels. Compared with baseline (II + III + Q/II + III), the increase was 2.47 times higher in older patients compared with young patients (6.5 ± 2.0 and 2.62 ± 0.83, respectively).
Coenzyme Q10-dependent MRC activity in GC reduces with aging. This reduction is diminished upon in vitro CoQ1 supplementation, indicating that CoQ10 deficit is the underlying cause for the mitochondrial dysfunction. The results show that functional CoQ10 status can be assessed by measuring complex II + III activity in GC and might provide a useful monitoring tool for future clinical studies of oral CoQ10 supplementation to older patients undergoing IVF treatment.
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