Total levels localization patterns and proportions of sperm exhibiting phospholipase C zeta are significantly correlated with fertilization rates after intracytoplasmic sperm injection

Total levels, localization patterns, and proportions of sperm exhibiting phospholipase C Zeta are significantly correlated with fertilization rates after intracytoplasmic sperm injection, but not after in vitro fertilization.

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Authors

Suseela Yelumalai, Ph.D., Marc Yeste, Ph.D., Celine Jones, Siti N. Amdani, M.Sc., Junaid Kashir, Ph.D., Ginny Mounce, M.Sc., Sarah J. Martins Da Silva, Ph.D., Christopher L. Barratt, Ph.D., Enda McVeigh, M.D., Kevin Coward, Ph.D.

Volume 104, Issue 3, Pages 561-568

Abstract

Objective:

To study the relationship of total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta, with fertilization rates after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).

Design:

Laboratory study; controls vs. patients after IVF (n = 27) or ICSI (n = 17) treatment.

Setting:

Fertility center.

Patient(s):

A total of 44 semen samples, subjected to either IVF or ICSI treatment. Oocyte collection, ICSI or IVF, determination of sperm concentration and motility, and immunocytochemical analyses of phospholipase C zeta (PLCζ).

Intervention(s):

None.

Main Outcome Measure(s):

Percentages of sperm exhibiting PLCζ.

Result(s):

Significant positive correlation between ICSI fertilization rates and total levels, localization patterns, and the proportion (percentage) of sperm exhibiting PLCζ. Total levels, localization patterns, and the proportion of sperm exhibiting PLCζ are correlated with fertilization rates for ICSI, but not for IVF.

Conclusion(s):

Evaluating total levels, localization patterns, and proportions of PLCζ may represent a useful diagnostic tool for clinical purposes in men for whom IVF is not advised or has previously failed. This clinical study further supports the fundamental role of PLCζ in the oocyte activation process.

Read the full text at: http://www.fertstert.org/article/S0015-0282(15)00365-9/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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