MicroRNA-451 plays a role in murine embryo implantation through targeting Ankrd46 as implicated by a microarray based analysis

miR-451 is specifically up-regulated during the implantation period and affects embryo implantation by conversely regulating Ankrd46, implicated by a microarray-based analysis.

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Zhengyu Li, M.D., Jia Jia, M.B.B.S., Jinhai Gou, M.B.B.S., Xia Zhao, M.D., Tao Yi, Ph.D.

Volume 103, Issue 3, Pages 834-844



To determine the potential microRNA (miRNA) regulators of embryo implantation, as a continuation of genomic and proteomic research.


Laboratory animal research.


University hospital laboratory.


Adult healthy female C57BL6/J mice (age 6–8 weeks, nonfertile, weighing 18–20 g each).


Female mice were mated naturally with fertile males to produce pregnancy. Luminal epithelium was collected by laser-capture microdissection during the implantation period. Mouse models of pseudopregnancy, delayed implantation, and artificial decidualization were established.

Main outcome measure(s):

The miRNA profile in luminal epithelium was clarified by microarray analysis and validated by real-time reverse transcription polymerase chain reaction (qRT-PCR) in a series of models. Target genes were predicted and confirmed by luciferase activity assay. The role of miRNA in implantation was examined by loss-of-function and gain-of-function of miRNA in vitro and in vivo.


A total of 29 and 15 miRNAs were up- and down-regulated, respectively, during the implantation period; 11 of these miRNAs were validated by qRT-PCR. The profile of miR-451 was clarified in a series of models. A dual-luciferase activity assay showed that Ankrd46 was a target gene of miR-451. Loss-of-function by LV-miR-451 sponge or miR-451 inhibitor led to a reduced number of embryo implantations, but had little effect on fertilization.


miR-451 was specifically up-regulated during the implantation period, and it may play a major role in embryo implantation by targeting Ankrd46.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)02380-2/fulltext

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.