MicroRNA 22-3p is down-regulated in the plasma of Han Chinese patients with premature ovarian failure

MiR-22-3p showed a lower expression level in 140 premature ovarian failure (POF) patients and was modestly effective in distinguishing POF from control subjects. It may be a consequence of the pathologic process of POF.

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Authors

Yujie Dang, M.D., Ph.D., Shidou Zhao, Ph.D., Yingying Qin, M.D., Ph.D., Ting Han, M.D., Ph.D., Weiping Li, M.D., Ph.D., Zi-Jiang Chen, M.D., Ph.D.

Volume 103, Issue 3, Pages 802-807

Abstract

Objective:

To determine whether plasma microRNAs (miRNAs) are differentially expressed between women with and without premature ovarian failure (POF), and to uncover the association of miRNAs with risk of POF.

Design:

Microarray with real-time polymerase chain reaction validation.

Setting:

University hospital.

Patient(s):

A total of 140 individuals with premature ovarian failure (POF) and 140 age- and body mass index–matched control subjects of Han Chinese ancestry.

Intervention(s):

None.

Main Outcome Measure(s):

Relative miRNA expression levels in plasma of POF and control group.

Result(s):

Fifty-one differentially expressed miRNAs were identified by chip-based discovery stage between ten patients with POF and ten control subjects, among which nine miRNAs (let-7b-5p, let-7c, miR-15b-5p, miR-22-3p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-151a-5p, and miR-151b) were selected and validated. The relative expression level of miR-22-3p was significantly down-regulated in POF compared with control subjects. MiR-22-3p yielded a receiver operating characteristic (ROC) curve area of 0.668 (95% confidence interval 0.602–0.733) in discriminating POF from controls. In addition, logistic binary regression analysis and linear regression analysis showed the miR-22-3p to be a protective factor for POF (odds ratio 0.766, 95% CI 0.643–0.912) and negatively associated with serum FSH. Furthermore, bioinformatics analysis indicated that the target function of miR-22-3p was involved in apoptosis, endocytosis, and tumorigenesis.

Conclusion(s):

Mir-22-3p showed a lower expression level in POF and was modestly effective in distinguishing POF from control subjects. The decreased expression of miR-22-3p in plasma of POF may reflect the diminished ovarian reserve and be a consequence of the pathologic process of POF.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)02535-7/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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