Longitudinal assessment of circulating insulin-like peptide 3 levels in healthy peripubertal girls

Immunohistochemistry and intraindividual variation, as well as relations to other ovarian hormones, reveal that INSL3 is a unique and specific marker of theca cells of antral follicles.

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Authors

Casper P. Hagen, Ph.D., Mikkel G. Mieritz, M.D., John E. Nielsen, M.Sc., Ravinder Anand-Ivell, Ph.D., Richard Ivell, Ph.D., Anders Juul, D.M.Sc.

Volume 103, Issue 3, Pages 780-786

Abstract

Objective:

To elucidate the natural course of circulating insulin-like peptide 3 (INSL3) levels according to puberty as well as its relation to other reproductive hormones.

Design:

Population-based cohort study.

Setting:

Not applicable.

Patient(s):

Healthy peripubertal girls (n = 10) examined every 6 months; total number of examinations was 84; median (range) per girl: 9 (4–10), including staging of pubertal breast development and blood samples.

Intervention(s):

None.

Main Outcome Measure(s):

Serum levels of INSL3, inhibin B, E2, antimüllerian hormone, LH, and FSH (validated immunoassays), and T and androstenedione (liquid chromatography–tandem mass spectrometry).

Result(s):

Serum levels of INSL3 varied considerably between girls (range, 0.01–0.27 ng/mL) and within each girl as puberty progressed; intraindividual variation, median (range) 102% (65%–143%). Insulin-like peptide 3 increased in late puberty (B1 to B4+B5); geometric mean 0.03 ng/mL to 0.15 ng/mL. Insulin-like peptide 3 levels reflected markers of large follicles (T, androstendione, inhibin B, and E2) better than markers of small follicles (antimüllerian hormone), and INSL3 staining was localized in theca interna cells of antral follicles.

Conclsion(s):

Insulin-like peptide 3 increased in late puberty, albeit inter- and intraindividual variations were substantial. Immunohistochemistry and intraindividual variation, as well as relations to other ovarian hormones, reveal that INSL3 in girls is a unique and specific marker of theca cells surrounding antral follicles. The potential clinical use of INSL3 for evaluation of ovarian function in girls remains to be elucidated.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)02360-7/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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