Antimüllerian hormone regulates stem cell factor expression in human granulosa cells

Antimullerian hormone regulates stem cell factor via the cyclic adenosine 30:50 monophosphate/protein kinase A (cAMP/PKA) pathway in human granulosa cells.

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Authors

Rong Hu, Ph.D., Fei-miao Wang, M.D., Liang Yu, Ph.D., Yan Luo, M.D., Xin Wu, B.Sc., Juan Li, M.D., Xiao-mei Zhang, Ph.D., Sergio Oehninger, M.D., Ph.D., Silvina Bocca, M.D., Ph.D.

Volume 102, Issue 6, Pages 1742-1750

Abstract

Objective:

To determine whether there is a correlation between antimüllerian hormone (AMH) and stem cell factor (SCF) in serum, follicular fluid (FF), and granulosa cells (GCs), and to investigate a possible regulatory mechanism of AMH on SCF in human granulosa cells.

Design:

Prospective clinical and experimental study.

Setting:

Academic center.

Patient(s):

163 women undergoing IVF.

Intervention(s):

Serum, FF, and GCs obtained in all women, primary cultures of human GCs.

Main Outcome Measure(s):

AMH and SCF were analyzed in serum, FF, and GCs, using enzyme-linked immunosorbent assay, reverse-transcription polymerase chain reaction, and immunoblotting.

Result(s):

There was a significant negative correlation between AMH and SCF protein level in FF, and in the mRNA expression of AMH and SCF in GCs. Conversely, there was no correlation between AMH and SCF levels in serum. In primary cultures of human GCs, SCF was down-regulated by treatment with recombinant human AMH and was increased by cyclic adenosine 3′:5′ monophosphate (cAMP) in a dose-dependent manner. A protein kinase A (PKA) inhibitor (H89) significantly reversed the effects of recombinant human AMH and cAMP on SCF mRNA and protein expression.

Conclusion(s):

This is the first report on a modulatory role for AMH as an ovarian/follicular autocrine/paracrine factor controlling SCF expression via the cAMP/PKA pathway.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)02071-8/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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