Pregnancy after allogeneic uterus transplantation in the rat Perinatal outcome and growth trajectory

Normal offspring and perinatal outcome can be achieved after allogeneic uterus transplantation.

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Authors

César Díaz-García, M.D., Liza Johannesson, M.D., Ph.D., Ruijin Shao, Ph.D., Håkan Bilig, M.D., Ph.D., Mats Brännström, M.D., Ph.D.

Volume 102, Issue 6, Pages 1545-1552

Abstract

Objective:

To investigate whether allogeneic uterine grafts in a rat model, with tacrolimus immunosuppression, can harbor pregnancies that result in offspring with normal postnatal growth.

Design:

Experimental animal study.

Setting:

Obstetrics and gynecology department at a university hospital.

Animal(s):

Lewis rats as uterus donors and Piebald-Virol-Glaxo rats as recipients.

Intervention(s):

Animals were allocated to one of the following three groups: allogeneic uterus transplantation with end-to-side anastomosis to the external iliac vessels and immunosuppression with tacrolimus (UT+Tac; n = 10); sham surgery and immunosuppression with tacrolimus (Sham+Tac; n = 10); or sham surgery (Sham; n = 10). The rats were subsequently introduced to male rats with proven fertility and in the event of resulting pregnancy cesarean sections were performed on day 22 of pregnancy.

Main Outcome Measure(s):

Graft viability, fertility rate, perinatal death, birth weight, postnatal birth trajectory.

Result(s):

Pregnancy rate was higher in the control groups (70% Sham and 80% Sham+Tac) than in the transplanted group (50% UT+Tac), although these differences did not reach the significance threshold. There were no differences between groups regarding number of living pups or neonatal deaths. Pups born from UT+Tac mothers had birth weights similar to external control animals from our breeding colony (BC): UT+Tac males 6.2 ± 0.2 g, UT+Tac females 5.5 ± 0.6 g, BC males 5.8 ± 0.2 g, BC females 5.2 ± 0.3 g; n.s. Evaluation of uteri and placentas of pregnant animals revealed a somewhat reduced vascular density in both tissues in the UT+Tac group, and that was not seen in the Sham+Tac group.

Conclusion(s):

Allogeneic uterus transplantation and immunosuppression with tacrolimus is compatible with normal pregnancy and perinatal outcome in a rat model.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)02162-1/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.