Timing the window of implantation by nucleolar channel system prevalence matches the accuracy of the endometrial receptivity array

We demonstrate that nucleolar channel systems are present during the window of endometrial receptivity previously identified by their transcriptomic signature using the endometrial receptivity array.

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Authors

Edward J. Nejat, M.D., M.B.A., Maria Ruiz-Alonso, M.Sc., Carlos Simón, M.D., Ph.D., U. Thomas Meier, Ph.D.

Volume 102, Issue 5, Pages 1477–1481

Abstract

Objective:

To test if nucleolar channel system (NCS) prevalence matches the accuracy of the endometrial receptivity array (ERA) for identification of the window of endometrial receptivity.

Design:

Comparative retrospective study, May 2008–May 2012.

Setting:

University-affiliated infertility clinic.

Patient(s):

49 healthy oocyte donors, regularly cycling, aged 20–34 years with a body mass index of 19–25 kg/m2.

Intervention(s):

Endometrial biopsies were collected throughout the menstrual cycle. All samples underwent transcriptomic signature identification by ERA testing (performed in a prior study) and quantification of NCS prevalence by using indirect immunofluorescence (performed in the present study).

Main Outcome Measure(s):

Concordance of ERA results determining the window of implantation with NCS prevalence was statistically analyzed using the kappa index. Based on dating according to the luteinizing hormone surge, specimens were dichotomized into receptive (n = 24) and nonreceptive (n = 25). The NCS prevalence was expressed as percentage of NCSs per endometrial epithelial cells in each endometrial biopsy.

Result(s):

Concordance of ERA and NCS dating vs. luteinizing hormone yielded comparable kappa indices of 0.878 and 0.836, respectively. Direct comparison of ERA and NCS dating resulted in a kappa index of 0.796.

Conclusion(s):

Prevalence of NCS identifies the window of endometrial receptivity previously identified by their transcriptomic signature using the ERA.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)02025-1/fulltext


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Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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