Development of a simplified method of human semen storage for the testing of sperm DNA fragmentation using the Halosperm G2 test kit

Human semen air-dried before DNA fragmentation analysis provides statistically significantly closer results to fresh semen compared with snap-frozen semen and can be stored for up to 1 month.

Like Comment


Ashleigh McEvoy, B.Sc., Peter Roberts, Ph.D., Kailin Yap, B.Sc.Hons., Phillip Matson, Ph.D.

Volume 102, Issue 4, Pages 981-988



To develop a simple, convenient, and stable storage method for semen before DNA fragmentation testing.


Experimental cross-sectional study.


Fertility clinic.


164 male partners of infertile couples.


Comparison of sperm DNA fragmentation levels (DFLs) using fresh, snap-frozen and air-dried semen, with air-dried samples stored at different temperatures and time periods to assess DNA stability.

Main Outcome Measure(s):

DFL determined by Halosperm G2 kit.


Results are expressed as mean ± standard error of the mean. The DFLs from fresh and air-dried semen gave comparable results (1.08% ± 0.65%), and from snap-frozen and fresh samples a statistically significant difference (5.5% ± 1.09%). Air-dried semen stored at room temperature for 7 days had a statistically significantly higher DFL compared with semen stored overnight (46.29% ± 9.12%). Samples stored at 4°C for 7 days or 1 day showed no statistically significant difference (0.83% ± 0.82%). DFLs from samples stored for either 1 or 30 days at 4°C showed a statistically significant difference (19.59% ± 5.72%); those stored at −22°C showed no statistically significant difference (0.68% ± 0.53%).


Air-drying semen is a simple and stable storage method for up to 1 month at −22°C before DNA fragmentation testing.

Read the full text at:

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

No comments yet.