Development of a simplified method of human semen storage for the testing of sperm DNA fragmentation using the Halosperm G2 test kit

Human semen air-dried before DNA fragmentation analysis provides statistically significantly closer results to fresh semen compared with snap-frozen semen and can be stored for up to 1 month.

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Ashleigh McEvoy, B.Sc., Peter Roberts, Ph.D., Kailin Yap, B.Sc.Hons., Phillip Matson, Ph.D.

Volume 102, Issue 4, Pages 981-988



To develop a simple, convenient, and stable storage method for semen before DNA fragmentation testing.


Experimental cross-sectional study.


Fertility clinic.


164 male partners of infertile couples.


Comparison of sperm DNA fragmentation levels (DFLs) using fresh, snap-frozen and air-dried semen, with air-dried samples stored at different temperatures and time periods to assess DNA stability.

Main Outcome Measure(s):

DFL determined by Halosperm G2 kit.


Results are expressed as mean ± standard error of the mean. The DFLs from fresh and air-dried semen gave comparable results (1.08% ± 0.65%), and from snap-frozen and fresh samples a statistically significant difference (5.5% ± 1.09%). Air-dried semen stored at room temperature for 7 days had a statistically significantly higher DFL compared with semen stored overnight (46.29% ± 9.12%). Samples stored at 4°C for 7 days or 1 day showed no statistically significant difference (0.83% ± 0.82%). DFLs from samples stored for either 1 or 30 days at 4°C showed a statistically significant difference (19.59% ± 5.72%); those stored at −22°C showed no statistically significant difference (0.68% ± 0.53%).


Air-drying semen is a simple and stable storage method for up to 1 month at −22°C before DNA fragmentation testing.

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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.