Up-regulation of heme oxygenase-1 expression modulates reactive oxygen species level during the cryopreservation of human seminiferous tubules

The ROS declined and HO-1 increased after the thawing of human seminiferous tubules. Slow freezing is more effective than vitrification in terms of HO-1 up-regulation and ROS decrease.

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Authors

Wenhao Tang, M.D., Jie Yan, Ph.D., Tianren Wang, Ph.D., Xi Xia, Ph.D., Xinjie Zhuang, Ph.D., Kai Hong, M.D., Rong Li, M.D., Ping Liu, M.D., Hui Jiang, M.D., Jie Qiao, M.D.

Volume 102, Issue 4, Pages 974-980

Abstract

Objective:

To study the effect of freezing techniques and to optimize a method for trace amounts of testicular spermatozoa from biopsed seminiferous tubules. The level of reactive oxygen species (ROS) and the gene expression of heme oxygenase-1 was evaluated.

Design:

Prospective experimental study.

Setting:

University-based laboratory.

Patient(s):

Eighteen adults with male fator infertility underwent testicular biopsy surgery.

Intervention(s):

Seminiferous tubular fragments from each man were evenly allocated to three groups: fresh control, slow freezing, and vitrifiaction groups. The morphology and ROS levels before and after freezing were evaluated for seminiferous tubular fragments. The expression of heme oxygenase-1 (HO-1) at both the transcriptional and protein levels was determined.

Main Outcome Measure(s):

The morphology was analyzed by light microscopy. The ROS levels were measured with ELISA. The proliferation and differentiation were evaluated by immunohistochemistry, and the expression of HO-1 was evaluated using a real-time polymerase chain reaction (PCR) and Western blotting.

Result(s):

Decreased ROS levels and increased HO-1 expression at the transcriptional and protein levels were observed after thawing the human seminiferous tubules. The ROS level was negatively correlated with HO-1 expression. Slow freezing was more effective than vitrification in terms of HO-1 up-regulation and ROS alteration.

Conclusion(s):

Based on our study, the slow freezing technique was more effective compared with the vitrification method.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)01363-6/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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