Human oocyte derived sperm chemoattractant is a hydrophobic molecule associated with a carrier protein

Treating oocyte-conditioned media with proteases and hexane provided evidence for the oocyte derived chemoattractant being a hydrophobic nonpeptide molecule that, in an oocyte-conditioned medium, is associated with a carrier protein.

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Authors

Leah Armon, Ph.D., Ido Ben‐Ami, M.D., Ph.D., Raphael Ron-El, M.D., Michael Eisenbach, Ph.D.

Volume 102, Issue 3, Pages 885-890

Abstract

Objective:

To characterize the nature of the human oocyte-derived chemoattractant.

Design:

Laboratory in vitro study.

Setting:

Academic research institute.

Patient(s):

Ten healthy sperm donors. Oocyte-conditioned media from women undergoing IVF treatment because of male factor infertility.

Intervention(s):

Sperm samples were processed by the migration-sedimentation technique. Oocyte-conditioned media were collected 2–3 hours after oocyte stripping.

Main Outcome Measure(s):

Sperm chemotaxis was assayed in a μ-slide chamber according to the direction of swimming relative to that of the chemical gradient.

Result(s):

Oocyte-conditioned media treated with proteases did not lose their chemotactic activity; on the contrary, they became more active, with the activity shifted to lower concentrations. When oocyte-conditioned media were subjected to hexane extraction, chemotactic activity was found in both the hydrophobic and aqueous phases. Known mammalian sperm chemoattractants were ruled out as oocyte-derived chemoattractants.

Conclusion(s):

Our results suggest that the oocyte-derived chemoattractant is a hydrophobic nonpeptide molecule that, in an oocyte-conditioned medium, is associated with a carrier protein that enables its presence in a hydrophilic environment.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)00544-5/fulltext


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Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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