Authors

Cihan Kaya, M.D., Hürriyet Turgut, M.D., Hüseyin Cengiz, M.D., Ayşenur Turan, C.N.M., Murat Ekin, M.D., Levent Yaşar, M.D.

Volume 102, Issue 3, Pages 878-884

Abstract

Objective:

To investigate the effect of enoxaparin on ovarian reserve and serum antimüllerian hormone (AMH) levels in a rat ovarian torsion model.

Design:

Experimental study.

Setting:

Experimental surgery laboratory in a training and research hospital.

Animal(s):

Fourteen female Wistar Hannover rats.

Intervention(s):

1) Control group received no special treatment other than abdominal exposure; 2) detorsion-only group received bilateral adnexal torsion (3-hour ischemia), and then after 3-hour torsion period, detorsion (reperfusion) was performed; and 3) detorsion-enoxaparin group received 0.5 mg/kg enoxaparin subcutaneously 2 hours before the same surgery as the detorsion-only group and a second 0.5 mg/kg dose of enoxaparin 24 hours after the first surgeries. Apart from the surgeries, preoperative and postoperative 1-mL blood samples were drawn from the right jugular vein of each rat.

Main Outcome Measure(s):

Preoperative and postoperative serum AMH levels, histopathologic damage scores, and follicle counts in the ovarian tissue of the rats.

Result(s):

Vascular congestion and hemorrhage scores were higher in the detorsion-enoxaparin group than in the detorsion-only and control groups. The number of small antral follicles was smaller in the detorsion-only group than in the control group. The difference in the pre- and postoperative AMH levels was higher in the detorsion-only group than in the control and detorsion-enoxaparin groups.

Conclusion(s):

The combination of enoxaparin therapy with conventional ovarian detorsion is more effective in protecting the ovarian reserve than detorsion alone.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)00540-8/fulltext