Telomere homeostasis is compromised in spermatocytes from patients with idiopathic infertility

The telomeric distribution of both the noncoding telomeric RNA (TERRA) and the protein component of telomerase (TERT) is disrupted in spermatocytes I of men with idiopathic infertility.

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Authors

Rita Reig-Viader, M.Sc., Laia Capilla, M.Sc., Marta Vila-Cejudo, M.Sc., Ferrán Garcia, M.D., Begoña Anguita, Ph.D., Montserrat Garcia-Caldés, Ph.D., Aurora Ruiz-Herrera, Ph.D.

Volume 102, Issue 3, Pages 728-738

Abstract

Abstract

Objective:

To study whether the telomere structure of germ cells from idiopathic infertile men is altered and if this impairment is influenced by meiotic recombination and telomere length.

Design:

We performed a detailed analysis of both telomeric repeat-containing RNA (TERRA) and telomerase distribution in testis cell spreads by combining immunofluorescence and RNA fluorescent in situ hybridization. In addition we analyzed meiotic recombination between homologous chromosomes by immunofluorescence and telomere length by quantitative fluorescent in situ hybridization.

Setting:

University.

Patient(s):

Men consulting for fertility problems.

Intervention(s):

Unilateral testicular biopsies.

Main Outcome Measure(s):

We observed that TERRA levels and its nuclear distribution were compromised in infertile patients. In addition, the presence of the protein component of telomerase at telomeres decreased in the affected patients. However, neither telomerase-TERRA association nor telomere length was altered in spermatocytes I of infertile samples compared with control individuals. In addition, we observed that meiotic recombination was reduced in infertile individuals.

Result(s):

Telomere homeostasis is impaired in infertile patients, and this was translated into a decrease in TERRA levels together with an alteration of the TERRA-protein component of telomerase telomeric association in primary spermatocytes.

Conclusion(s):

This study demonstrates for the first time that telomere structure and homeostasis in germ cells is compromised in infertile individuals. In the light of our results we propose that the analysis of telomeric structure (i.e., TERRA levels and telomere association with TERRA and telomerase) would provide new tools for our understanding of the origin of human infertility.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)00538-X/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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