Influence of ejaculatory abstinence on seminal total antioxidant capacity and sperm membrane lipid peroxidation

A single day of ejaculatory abstinence (EA) compared with 4 days of EA increased seminal total antioxidant capacity without a measurable effect on sperm membrane lipid peroxidation.

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Authors

Paul Marshburn, M.D., Allie Giddings, M.D., Stephanie Causby, M.Sc., Michelle L. Matthews, M.D., Rebecca S. Usadi, M.D., Nury Steuerwald, Ph.D., Bradley S. Hurst, M.D.

Volume 102, Issue 3, Pages 705-710

Abstract

Objective:

To determine whether the period of ejaculatory abstinence (EA) influences the total antioxidant capacity (TAC) of semen or lipid peroxidation (LPO) of sperm membranes.

Design:

A prospective experimental trial.

Setting:

Academic medical center for reproductive endocrinology and infertility.

Patient(s):

Forty men from infertile couples planning intrauterine insemination.

Intervention(s):

Men provided semen specimens after EA periods of 1 and 4 days.

Main Outcome Measure(s):

Semen analysis, peroxidase staining, and assays for seminal TAC and sperm membrane LPO, with measures compared between days 1 and 4 within individuals (internal control) using paired t tests.

Result(s):

The shorter period of EA (1 day vs. 4 days) resulted in statistically significant decreases in semen volume (−24%), sperm density (−28%), and total sperm count (−3.2%). There was a statistically significant increase in TAC with the shorter period of EA (1 day) compared with 4 days of EA. No difference was detected in sperm membrane LPO comparing 1 day of EA and 4 days of EA.

Conclusion(s):

Higher seminal TAC obtained after a shorter period of EA could diminish oxidative stress–induced sperm damage by a mechanism independent of LPO. Shorter periods of EA may thus improve sperm quality by protecting from reactive oxygen species damage, even though lower numbers of motile sperm are produced after a shorter period of EA. This would be consistent with prior research indicating improved results after intrauterine insemination under these circumstances.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)00502-0/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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