Sperm involved in recurrent partial hydatidiform moles cannot induce the normal pattern of calcium oscillations

Sperm involved in recurrent partial hydatidiform mole pregnancies is not able to activate human oocytes or trigger the normal pattern of Ca2+ oscillations in mouse and human oocytes in vitro.

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Authors

Dimitra Nikiforaki, M.Sc., Frauke Vanden Meerschaut, M.D., Ph.D., Stefanie De Gheselle, M.Sc., Chen Qian, M.D, Etienne Van den Abbeel, Ph.D., Winnok H. De Vos, Ph.D., Tom Deroo, Ph.D., Petra De Sutter, M.D., Ph.D., Björn Heindryckx, Ph.D.

Volume 102, Issue 2, Pages 581–588.e1

Abstract

Objective:

To assess the Ca2+-releasing ability of sperm involved in partial hydatidiform moles.

Design:

Analysis of the activating and Ca2+-releasing ability of human sperm.

Setting:

University hospital research laboratory.

Patient(s):

Patients undergoing intracytoplasmic sperm injection (ICSI) treatment.

Intervention(s):

Microinjection of mouse and human oocytes with sperm.

Main Outcome Measure(s):

Measurement of the fertilizing and Ca2+-releasing ability of human sperm.

Result(s):

The mouse oocyte Ca2+ analysis showed that only 19.0% (4/21) of the mouse oocytes injected with sperm involved in molar pregnancies exhibited a normal pattern of Ca2+ oscillations versus 63.2% (36/57) of those injected with control sperm. Further, 83.3% (15/18) of donated in vitro–matured human oocytes injected with deficient sperm did not exhibit any Ca2+ release, while 76.9% (10/13) failed to show normal pronuclear development. Yet the sperm oocyte activation factor phospholipase C zeta (PLCζ) was present in the majority (96.6%, n = 113) of the analyzed sperm at a normal expression level. Eventually, fertilization failure was overcome with assisted oocyte activation in subsequent therapeutic ICSI cycles, which led to normal deliveries.

Conclusion(s):

Sperm that previously provoked recurrent partial hydatidiform mole pregnancies due to dispermic fertilization is not able to activate human oocytes or trigger the normal pattern of Ca2+ oscillations in mouse and human oocytes in vitro.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)00416-6/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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