In the secretory endometrium of women luminal epithelia exhibit gene and protein expressions that differ from those of glandular epithelia

The function of luminal epithelia appears to differ from that of glandular epithelia, even though both cell types are continuous in the functionalis layer of the endometrium.

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Authors

Gloria E. Evans, Ph.D., Gregory T. Phillipson, M.B.Ch.B., Peter H. Sykes, M.B.Ch.B., Iris L. Sin, Ph.D., Enid Y. Lam, Ph.D., Cristin G. Print, M.B.Ch.B., Ph.D., José A. Horcajadas, Ph.D., John J. Evans, Ph.D.

Volume 102, Issue 1, Pages 307–317.e7

Abstract

Objective:

To characterize the transcriptome of luminal epithelia (LE) of fertile secretory endometria and compare the results with those from glandular epithelia (GE).

Design:

Endometrial samples were collected at 2 and 7 days after initial blood LH surge in separate menstrual cycles. LE were obtained with the use of laser microdissection. mRNA was amplified with the use of linear polymerase chain reaction and hybridized to Agilent 4×44 microarrays. Gene analysis was used to identify differentially expressed mRNAs. Immunohistochemistry was used to assess nine proteins.

Setting:

One IVF clinic.

Patient(s):

Seven Caucasian fertile cycling women.

Intervention(s):

None.

Main Outcome Measure(s):

Cycle dating with the use of blood endocrinologic markers, microarrays of laser-microdissected LE, immunohistochemical analysis.

Result(s):

One hundred sixty-one (of 401) differentially expressed mRNAs in LE were identified from the metabolism pathway. Increased selective protein expression in LE at 7 days after initial LH surge was observed. LE mRNA expression was the converse of that in GE. The two cell types each had a different significant biologic pathway identified.

Conclusion(s):

Our results introduce a new concept that LE differentially expressed mRNAs are in the converse direction to that of GE, indicating different biologic processes despite the GE being continuous with the luminal monolayer. This probable distinction of biologic roles has not been noted previously. Further investigations must take cognizance of this observation.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)00350-1/fulltext


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Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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