Micro-electrophoresis: A noninvasive method of sperm selection based on membrane charge

Analysis of sperm membrane charge using microelectrophoresis demonstrates that semen samples with a higher proportion of negatively charged sperm are associated with improved ART outcomes.

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Authors

Luke Simon, Ph.D., Kristin Murphy, Ph.D., Kenneth I. Aston, Ph.D., Benjamin R. Emery, M.Phil., James M. Hotaling, M.D., Douglas T. Carrell, Ph.D.

Volume 103, Issue 2, Pages 361-366

Abstract

Objective:

To develop a technique with the potential of isolating genetically fit sperm for assisted reproductive technology (ART) treatment without compromising its structural or functional competence.

Design:

Observational study.

Setting:

University hospital.

Patient(s):

Fifty patients undergoing infertility diagnosis and 88 couples undergoing ART treatment.

Intervention(s):

None.

Main Outcome Measure(s):

Under an electric field, the percentage of positively charged sperm (PCS), negatively charged sperm (NCS), and neutrally charged sperm was determined for each ejaculate before and after density gradient centrifugation (DGC), and evaluated for sperm DNA damage, histone retention, and couples’ ART outcomes. Subsequently, PCS, NCS, and neutrally charged sperm were selected using an intracytoplasmic sperm injection needle and directly analyzed for DNA damage.

Result(s):

There was a reduction in the NCS population (95.10% ± 0.94% vs. 54.48% ± 2.39%) and an increase in the PCS population (4.28% ± 0.58% vs. 42.52% ± 2.36%) after DGC. The DNA damage was inversely proportional to %NCS (r2 = −0.242) and directly proportional to the %PCS (r2 = 0.206). When sperm were picked according to their charge and directly analyzed, sperm DNA damage was lower in the NCS population (3.9% ± 1.5%) compared with control (17.3% ± 3.2%) and %PCS populations (27.8% ± 6.0%). The %NCS was positively associated with fertilization rate (r2 = 0.469) and blastocyst development (r2 = 0.308) and inversely associated with embryo arrest (r2 = −0.253). Implantation rate and clinical pregnancies were higher in patient groups with increased NCS.

Conclusion(s):

Selection of NCS through micro-electrophoresis has the potential to isolate sperm relatively free of DNA damage to be used in ART.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)02307-3/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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