Ying Wang, B.S., Yuan Zhang, B.S., Ming-Qing Li, Ph.D., M.D., Deng-Xuan Fan, M.S., Xiao-Hui Wang, B.S., Da-Jin Li, M.D., Ph.D., Li-Ping Jin, M.D., Ph.D.
Volume 102, Issue 1, Pages 257–263
To clarify the role and mechanism of interleukin (IL)-25 in regulating the biological functions of decidual stromal cells (DSCs) in human early pregnancy.
Laboratory study of the effect of IL-25 induced by hCG on the proliferation of DSCs.
Women aged 23–47 years with normal pregnancy and abortion.
Main Outcome Measure(s):
Signal transduction from IL-25.
Here we show that DSCs coexpress IL-25/IL-17RB. Human chorionic gonadotropin promotes the expression of IL-25/IL-17RB. In contrast to anti-human IL-25 neutralizing antibody, recombinant human IL-25 (rhIL-25) significantly stimulates the proliferation of DSCs in dosage- and time-dependent manners. RhIL-25 promotes the phosphorylation of AKT, extracellular-signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinases (JNK), and nuclear factor κB in DSCs. Interestingly, blocking JNK or AKT signal with inhibitors down-regulates the stimulatory effect on DSC proliferation induced by rhIL-25. In addition, the results of Western blot show that the expression of IL-25/IL-17RB in DSCs from normal pregnancy was higher than that from abortion.
Our results have revealed that hCG derived of trophoblasts up-regulates the expression of IL-25/IL-17RB in DSCs and that IL-25 further stimulates the proliferation of DSCs through activating JNK and AKT signals, which finally contributes to the establishment and maintenance of physiological pregnancy.
Read the full text at: http://www.fertstert.org/article/S0015-0282(14)00266-0/fulltext