Interleukin 25 induced by human chorionic gonadotrophin promotes the proliferation of decidual stromal cells by activation of JNK and AKT signal pathways

Interleukin-25 expression was enhanced by trophoblast-derived hCG, could stimulate the proliferation of DSCs through activating JNK and AKT signals, which finally contributes to the establishment and maintenance of physiological pregnancy.

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Authors

Ying Wang, B.S., Yuan Zhang, B.S., Ming-Qing Li, Ph.D., M.D., Deng-Xuan Fan, M.S., Xiao-Hui Wang, B.S., Da-Jin Li, M.D., Ph.D., Li-Ping Jin, M.D., Ph.D.

Volume 102, Issue 1, Pages 257–263

Abstract

Objective:

To clarify the role and mechanism of interleukin (IL)-25 in regulating the biological functions of decidual stromal cells (DSCs) in human early pregnancy.

Design:

Laboratory study of the effect of IL-25 induced by hCG on the proliferation of DSCs.

Setting:

Research laboratories.

Patient(s)

Women aged 23–47 years with normal pregnancy and abortion.

Intervention(s):

None.

Main Outcome Measure(s):

Signal transduction from IL-25.

Result(s):

Here we show that DSCs coexpress IL-25/IL-17RB. Human chorionic gonadotropin promotes the expression of IL-25/IL-17RB. In contrast to anti-human IL-25 neutralizing antibody, recombinant human IL-25 (rhIL-25) significantly stimulates the proliferation of DSCs in dosage- and time-dependent manners. RhIL-25 promotes the phosphorylation of AKT, extracellular-signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinases (JNK), and nuclear factor κB in DSCs. Interestingly, blocking JNK or AKT signal with inhibitors down-regulates the stimulatory effect on DSC proliferation induced by rhIL-25. In addition, the results of Western blot show that the expression of IL-25/IL-17RB in DSCs from normal pregnancy was higher than that from abortion.

Conclusion(s):

Our results have revealed that hCG derived of trophoblasts up-regulates the expression of IL-25/IL-17RB in DSCs and that IL-25 further stimulates the proliferation of DSCs through activating JNK and AKT signals, which finally contributes to the establishment and maintenance of physiological pregnancy.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)00266-0/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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