Urinary metabolome identifies signatures of oligozoospermic infertile men

The urinary metabolome could differentiate oligozoospermic infertile men from fertile men. The disrupted metabolic pathways reflected fertility reduction that may be tightly associated with energy consumption and antioxidant defenses in spermatogenesis.

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Authors

Jie Zhang, Ph.D., Zhenzhen Huang, Ph.D., Minjian Chen, Ph.D., Yankai Xia, Ph.D., Francis L. Martin, Ph.D., Wei Hang, Ph.D., Heqing Shen, Ph.D.

Volume 102, Issue 1, Pages 44–53.e12

Abstract

Objective:

To identify the differential urinary metabolic pattern of oligozoospermic infertile men and to determine the potential biomarkers indicative of infertility.

Design:

Observational study.

Setting:

University hospital.

Patient(s):

Totals of 158 fertile volunteers and 135 oligozoospermic infertile men.

Intervention(s):

None.

Main Outcome Measure(s):

Urinary metabolic profiles were acquired with the use of liquid chromatography/mass spectrometry. Potential oligozoospermic biomarkers were screened from orthogonal projections to latent structures discriminant analysis and further evaluated by receiver operating characteristic analysis. The Spearman correlations between the individual sets of biomarkers and between biomarkers and sperm parameters were investigated. The disrupted biologic pathways which the biomarkers were involved in also were analyzed.

Result(s):

Oligozoospermic infertile men could be differentiated from fertile control subjects based on altered urinary metabolic profiles. A total of ten potential biomarkers were screened and tentatively identified. Among those, decreased acylcarnitines, aspartic acid, and leucylproline and increased adenine and methylxanthine were strongly associated with oligozoospermic risk. Many biomarkers were associated with sperm concentration and amplitude of lateral head displacement. The combined pattern of acetylcarnitine, carnitine C3:1, and aspartic acid provided moderate diagnostic power.

Conclusion(s):

Urinary metabolomics identified unique metabolic pattern of oligozoospermic infertility. The potential biomarkers suggested that oligozoospermia may be tightly associated with energy consumption and antioxidant defenses in spermatogenesis.

Read the full text at: http://www.fertstert.org/article/S0015-0282(14)00288-X/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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