Genome wide profiling of long noncoding ribonucleic acid expression patterns in ovarian endometriosis by microarray

Genome-wide lncRNA expression patterns of ovarian endometriosis tissue are profiled by microarray, compared with paired eutopic endometrium. Nearly 1,000 lncRNA transcripts are found dysregulated.

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Authors

Peng-ran Sun, Ph.D., Shuang-zheng Jia, Ph.D., Hong Lin, M.D., Jinhua Leng, M.D., Jinghe Lang, M.D.

Volume 101, Issue 4, Pages 1038-1046.e7

Abstract

Objective:

To profile the long noncoding RNA (lncRNA) expression patterns in ovarian ectopic endometrial tissue compared with paired eutopic endometrial tissue.

Design:

Genome-wide expression analysis of human tissue.

Setting:

University hospital.

Patient(s):

Twenty-five patients receiving laparoscopic surgeries for ovarian endometriosis.

Intervention(s):

Ovarian ectopic endometrial tissue was obtained during surgery. Eutopic endometrial tissue was taken by curettage at the same time.

Main Outcome Measure(s):

Ectopic and eutopic endometrial lncRNA and messenger RNA (mRNA) expression levels were determined by microarray in four patients; quantitative reverse transcription–polymerase chain reaction validation of 10 differentially expressed lncRNAs was conducted in another 21 patients. The lncRNAs’ functions were predicted through coexpressed mRNA annotations.

Result(s):

A total of 948 lncRNA transcripts and 4,088 mRNA transcripts were dysregulated in ectopic endometrial tissue, compared with paired eutopic endometrial tissue. The expressions of the 10 chosen lncRNAs were validated by quantitative reverse transcription–polymerase chain reaction. Functional analysis suggests that several groups of lncRNAs may participate in biological pathways related to endometriosis by cis- and/or trans-regulation of protein-coding genes.

Conclusion(s):

This study constitutes the first report of lncRNA expression patterns in human ectopic and eutopic endometrial tissue. Nearly 1,000 dysregulated lncRNA transcripts are found by microarray.

Read the full text at: http://www.fertstert.org/article/S0015-0282(13)03467-5/fulltext


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Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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