Levels of the retinoic acid synthesizing enzyme ALDH1A2 are lower in testicular tissue from men with infertility

Levels of aldehyde dehydrogenase-1A2 (ALDH1A2), which synthesizes retinoic acid, are significantly reduced in testicular tissue from men with infertility compared with controls, suggesting an association between ALDH1A2 and male infertility.

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Authors

John K. Amory, M.D., M.P.H., Samuel Arnold, M.Sc., Maria C. Lardone, M.Sc., Antonio Piottante, M.D., Mauricio Ebensperger, M.D., Nina Isoherranen, Ph.D., Charles H. Muller, Ph.D., Thomas Walsh, M.D., Andrea Castro, M.Sc.

Volume 101, Issue 4, Pages 960-966

Abstract

Objective:

To determine whether decreased testicular levels of enzymes necessary for retinoic acid biosynthesis were associated with male infertility, as retinoic acid is known to be necessary for spermatogenesis.

Design:

Observational analysis of testicular tissue samples, sperm indices, and serum hormone concentrations.

Setting:

Two infertility centers in Chile.

Patient(s):

32 infertile men and 11 control men.

Intervention(s):

Measurement of the three enzymes necessary for retinoic acid biosynthesis, aldehyde dehydrogenase (ALDH) 1A1, 1A2, and 1A3, in testicular tissue by a novel liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) peptide assay.

Main Outcome Measure(s):

ALDH isozyme levels compared by type of infertility and correlated with testicular germ cell numbers, sperm parameters, and serum and intratesticular hormone concentrations.

Result(s):

Men with infertility had statistically significantly reduced levels of ALDH1A2 but not ALDH1A1 or ALDH1A3 in their testicular tissue compared with men with normal spermatogenesis. The ALDH1A2 protein levels were strongly correlated with the number of germ cells found via testicular biopsy.

Conclusion(s):

These findings suggest that ALDH1A2 is the enzyme involved in retinoic acid biosynthesis in human germ cells. Further study of the relationship between intratesticular ALDH1A2 and male infertility is warranted to determine whether men with infertility have a reduced ability to synthesize retinoic acid within their germ cells that could impair spermatogenesis.

Read the full text at: http://www.fertstert.org/article/S0015-0282(13)03489-4/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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