A randomized clinical trial of endometrial perfusion with granulocyte colony stimulating factor in in vitro fertilization cycles Impact on endometrial thickness and clinical pregnancy rates

Granulocyte colony-stimulating factor neither affects endometrial thickness nor pregnancy rates in normal in vitro fertilization patients.

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David H. Barad, M.D., M.S., Yao Yu, Ph.D., Vitaly A. Kushnir, M.D., Aya Shohat-Tal, Ph.D., Emanuela Lazzaroni, M.S., Ho-Joon Lee, Ph.D., Norbert Gleicher, M.D.

Volume 101, Issue 3, Pages



To investigate whether granulocyte colony-stimulating factor (G-CSG) affects endometrial thickness, implantation rates, and clinical pregnancy rates in routine, unselected IVF cycles.


Registered, individually randomized, two-group, parallel double-blinded placebo-controlled clinical trial.


Academically affiliated private clinical and research center.


141 consecutive, unselected, consenting women with no history of renal disease, sickle cell disease, or malignancy who were undergoing IVF.


Sealed, numbered, opaque envelopes assigned 73 patients to receive G-CSF (Filgrastim, Amgen, 300 μg/1.0 mL) and 68 to receive placebo (saline).

Main Outcome Measure(s):

Endometrial thickness, clinical pregnancy, and embryo implantation rates.


The mean age for the whole study group was 39.59 ± 5.56 years (G-CSF: 39.79 ± 5.13 years; placebo: 39.38 ± 6.03 years). Endometrial thickness statistically significantly increased over the 5-day observation period for the whole group by approximately 1.36 mm. The increase in the G-CSF group was not statistically significantly different from the control group. Statistical models looking at treatment effects on clinical pregnancy and implantation rates demonstrated no effect of G-CSF treatment. There were no adverse events for either treatment group.


In normal IVF patients, G-CSF does not affect endometrial thickness, implantation rates, or clinical pregnancy rates. Because these results were obtained in an older patient population, they may not necessarily apply to younger women.

Read the full text at: http://www.fertstert.org/article/S0015-0282(13)03435-3/fulltext

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.