Tao Gui, M.D., Chen Chen, M.D., Zhenzhen Zhang, M.D., Weiwei Tang, M.D., Ruyun Qian, M.D., Xiaoping Ma, M.D., Peng Cao, Ph.D., Guiping Wan, Ph.D.
Volume 101, Issue 2, Pages 506-514, February 2014
To investigate the balance between regulatory T cells (Treg) and T-helper 17 cells (TH17) in peripheral blood and uteri of women with adenomyosis (AM), and to evaluate their potential correlation with dysmenorrhea and CA-125 levels.
Laboratory study using human peripheral blood and tissues.
Forty-five patients with AM (study group) and 25 women without AM (control group).
The peripheral blood and tissues harvested from all groups were subjected to flow cytometry, ELISA, quantitative real-time polymerase chain reaction, and immunohistochemistry. The severity of dysmenorrhea was distinguished by visual analog scale (VAS).
Main Outcome Measure(s):
TH17 and Treg cell frequency, mRNA and protein levels of transcription factors and cytokines in all groups, and their correlation between the TH17-Treg ratio and dysmenorrhea severity or CA-125 level.
The disturbance of TH17-Treg balance was demonstrated in peripheral circulation and uteri of patients with both diffuse and focal AM, and it correlated positively with dysmenorrhea severity and CA-125.
The findings suggest that TH17-Treg imbalance may play a crucial role in the immunopathogenesis of AM, and may be thus a potential target of AM therapy.
Clinical Trial Registration Number:
Read the full text at: http://www.fertstert.org/article/S0015-0282(13)03213-5/fulltext