Coculturing human endometrial epithelial cells and stromal fibroblasts alters cell specific gene expression and cytokine production

A coculture system of polarized human endometrial epithelium and subject-paired stroma reveals effects of paracrine signaling on cell-specific global gene expression and cytokine production.

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Authors

Joseph C. Chen, Ph.D., M.S., David W. Erikson, Ph.D., Terhi T. Piltonen, M.D., Ph.D., Michelle R. Meyer, B.S., Fatima Barragan, B.S., Ramsey H. McIntire, Ph.D., John S. Tamaresis, Ph.D., Kim Chi Voa, B.S., Linda C. Giudice, M.D., Ph.D., M.Sc., Juan C. Irwina, M.D., Ph.D.

Volume 100, Issue 4, Pages 1132-1143, October 2013

Abstract

Objective:

To determine the effects of coculturing endometrial epithelial cells (eEC) with paired endometrial stromal fibroblasts (eSF) on cell-specific gene expression and cytokine secretion patterns.

Design:

In vitro study.

Setting:

University research laboratory.

Patient(s):

Endometrial biopsies were obtained from premenopausal women.

Intervention(s):

Polarized eEC and subject-paired eSF were cultured for 12.5 hours alone (monoculture) or combined in a two-chamber coculture system without cell-cell contact. Cells and conditioned media were analyzed for global gene expression and cytokine secretion, respectively. Purified, endometrial tissue-derived eEC and eSF isolated by fluorescent activated cell sorting (FACS) were used as noncultured controls.

Main Outcome Measure(s):

Cell-specific global gene expression profiling and analysis of secreted cytokines in eEC/eSF cocultures and respective monocultures.

Result(s):

Transepithelial resistance, diffusible tracer exclusion, expression of tight junction proteins, and apical/basolateral vectorial secretion confirmed eEC structural and functional polarization. Distinct transcriptomes of eEC and eSF were consistent with their respective lineages and their endometrial origin. Coculture of eEC with eSF resulted in altered cell-specific gene expression and cytokine secretion.

Conclusion(s):

This coculture model provides evidence that interactions between endometrial functionally polarized epithelium and stromal fibroblasts affect cell-specific gene expression and cytokine secretion underscoring their relevance when modeling endometrium in vitro.

Read the full text at: http://www.fertstert.org/article/S0015-0282(13)00701-2/fulltext


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Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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