Identification of a novel sperm motility–stimulating protein from caprine serum: its characterization and functional significance

A sperm forward motility–stimulating protein was purified from goat serum and characterized. The research provides a potential nonsteroidal biomolecule and its antibody that may solve some global reproductive problems.

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Authors

Sudipta Saha, Ph.D., Sujoy Das, M.Sc., Arpita Bhoumik, M.Sc., Prasanta Ghosh, M.Sc., Gopal Chandra Majumder, Ph.D., Sandhya Rekha Dungdung, Ph.D.

Volume 100, Issue 1, Pages 269-279.e5, July 2013

Abstract

Objective:

To identify and characterize a novel sperm motility–stimulating protein (MSP) from caprine serum.

Design:

Prospective experimental study.

Setting:

Research laboratory.

Animal(s):

Rabbits and male and female BALB/c mice.

Intervention(s):

Protein purification by conventional methods and functional and immunological characterization.

Main Outcome Measure(s):

MSP was purified, purity was checked, and molecular weight was determined. Surface localization, tissue distribution, and IVF for contraceptive efficacy were studied.

Result(s):

MSP is a heat-stable 66-kDa monomeric novel protein. At 0.9 μM, it showed much higher forward motility and longer motility maintenance than other known activators. Studies with the motility analyzers CASA and SPERMA showed an increase in horizontal and vertical velocities, respectively. MSP action was cyclic AMP independent. Its occurrence was higher in testis, although blood was the richest source. MSP was localized throughout the cell surface of spermatozoa. Its antibody caused significant inhibition of sperm motility and inhibited fertilization up to 100% at 1:25 dilution, which showed its contraceptive efficacy.

Conclusion(s):

MSP has high efficacy to stimulate sperm forward motility and thus may be used in biomedical application in infertility clinics, animal breeding centers, poultries, and animal conservation centers.

Read the full text at: http://www.fertstert.org/article/S0015-0282(13)00413-5/fulltext


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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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