Comparison of embryo morphokinetics after in vitro fertilization-intracytoplasmic sperm injection in smoking and nonsmoking women

The comparison of embryo morphokinetics after IVF-ICSI cycles according to female smoking status showed that early embryo development is impaired by female smoking, leading to poor IVF outcome.


Thomas Fréour, Pharm.D., M.Sc., Lionel Dessolle, M.D., M.Sc., Jenna Lammers, M.Sc., Stéphanie Lattes, M.Sc., Paul Barrière, M.D., Ph.D.

Volume 99, Issue 7, Pages 1944-1950, June 2013



To evaluate the impact of female smoking on early embryo development in vitro using a time-lapse system.


Retrospective analysis of prospectively collected database.


Assisted reproductive technology (ART) program.


A total of 135 infertile couples undergoing IVF-ICSI cycle.


Embryo culture in a time-lapse incubator (Embryoscope, Unisense Fertilitech).

Main outcome measure(s):

Embryo morphokinetic parameters.


Of 135 women, 139 and 729 oocytes were cultured in 23 smokers and 112 non-smokers, respectively. Basal characteristics and ovarian stimulation parameters were comparable in both groups. Serum AMH and Antral Follicle Count were significantly lower in smokers than in non-smokers. Conventional embryologic parameters were comparable in both groups, but time lapse analysis showed that most cleavage events occurred significantly later in smokers than in non-smokers, leading to poor cycle outcome in smokers.


Female smoking impairs early embryo development, as reflected by time-lapse analysis. This might contribute to the dramatic decrease in pregnancy rates in smokers undergoing IVF. Time lapse is a useful tool for the identification of deleterious components impairing early embryo development.

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