Prospective controlled study to evaluate laboratory and clinical outcomes of oocyte vitrification obtained in in vitro fertilization patients aged 30 to 39 years

In a sibling oocyte study conducted in infertile patients 30–39 years of age, oocyte vitrification had minimal negative impact on embryo development and subsequent clinical results.

Like Comment

Authors

Ching-Chien Chang, Ph.D., Thomas A. Elliott, B.Sc., Graham Wright, B.Sc., Daniel B. Shapiro, M.D., Andrew A. Toledo, M.D., Zsolt Peter Nagy, M.D., Ph.D.

Volume 99, Issue 7, Pages 1891-1897, June 2013

Abstract

Objective:

To determine whether the process of oocyte vitrification affects oocyte viability in in vitro fertilization (IVF) patients between 30 and 39 years of age.

Design:

Prospective controlled study.

Setting:

Private IVF practice.

Patient(s):

A total of 30 women assigned and 22 qualified.

Intervention(s):

Denudation of oocytes, cryopreservation of oocytes using vitrification method in a medium with 15% ethylene glycol (EG), 15% dimethylsulfoxide (DMSO), and 0.5 M sucrose.

Main Outcome Measure(s):

Oocyte survival, fertilization, day-3 embryo quality, blastocyst formation, clinical pregnancy, implantation, and live-birth rates.

Result(s):

After denudation of oocytes, mature sibling oocytes were randomly allocated to the fresh and vitrified groups. The survival rate was 79.6% after vitrification/warming. Overall, no statistically significant differences were found in fertilization, day-3 embryo quality, or blastocyst formation rates between the fresh and vitrified groups. The positive β-human chorionic gonadotropin, clinical pregnancy rate, and implantation rate were 13 (59.0%) of 22, 10 (45.4%) of 22, and 16 (30.1%) of 53 for the vitrified group. The overall efficiency in achieving a live birth was 11 (5.9%) of 186 per vitrified oocyte.

Conclusion(s):

The impact of vitrification can be reduced to a minimal level, making it possible to achieve high pregnancy and implantation rates in this age group of IVF patients.

Read the full text at: http://www.fertstert.org/article/S0015-0282(13)00265-3/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.