Effect of an isotonic lubricant on sperm collection and sperm quality

Use of isotonic lubricant is safe during collection of semen samples.

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Ashok Agarwal, Ph.D., Helena Malvezzi, M.S., Rakesh Sharma, Ph.D.

Volume 99, Issue 6, Pages 1581-1586, May 2013



To assess the influence of an isotonic lubricant used during sperm sample collection on [1] ease of collection and [2] resultant sperm quality.

Study Design:

Paired randomized cross-over design.


Tertiary Hospital.


Healthy men over 18 years old with normal semen analysis as per WHO 2010 guidelines.


Collection of semen sample by masturbation with or without the use of Pre-Seed personal lubricant.

Main Outcome Measures:

Qualitative survey results and quantitative sperm function outcomes were measured to determine resultant sperm quality and collection experience with and without Pre-Seed lubricant.


The qualitative questionnaire results showed that 73% of donors prefer the semen collection process with the isotonic lubricant and 55% recommended the use of lubricant in their everyday collection. The motility, viability, membrane integrity, levels of ROS, total antioxidant capacity and percentage of DNA damage in collected semen samples were not affected by the use of the lubricant.


More donors prefer, and find it easier, to collect semen samples with the use of the lubricant. The isotonic lubricant Pre-Seed did not compromise sperm quality as evaluated in an array of sperm assays, suggesting its safe use in fertility patients as required during sperm collection.

Read the full text at: http://www.fertstert.org/article/S0015-0282(13)00172-6/fulltext

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.