Authors
Ze-Xu Jiao, M.D., Ph.D. and Teresa K. Woodruff, Ph.D.
Volume 99, Issue 5, Pages 1453-1459.e1, April 2013
Abstract
Objective:
To determine whether the follicle environment modulates oocyte-specific gene transcript levels in cultured oocytes and polar bodies (PB).
Design:
Animal study.
Setting:
Large academic research center.
Animals:
CD1 mice.
Interventions:
In vitro growth of secondary mouse follicles in 0.25% or 1.5% alginate (ALG) in a three-dimensional culture system.
Main outcome measures:
Relative RNA levels of Gdf9, Bmp15, Nlrp5, Tcl1, and Zp3 measured by real-time quantitative RT-PCR in oocytes during in vitro follicle development and oocyte maturation and in their first polar bodies after removal from MII eggs.
Results:
All transcripts decreased earlier in oocytes cultured in 1.5% ALG compared with 0.25% ALG. Transcript levels were lower in MII eggs cultured in 1.5% ALG compared with 0.25% ALG. All genes were expressed in PBs; levels were lower in follicles cultured in 1.5% ALG compared with 0.25% ALG. Abundance of all transcripts was lower in PBs than in their sibling oocytes.
Conclusions:
Local follicle environment modulates oocyte-specific gene expression in the oocyte and first PB. There is a significant difference in the transcript levels of oocyte-specific genes in PB from 1.5% vs. 0.25% ALG that correlates with ovarian environment-related decreases in oocyte competence.
Read the full text at: http://www.fertstert.org/article/S0015-0282(12)02503-4/fulltext