A novel mouse model that closely mimics human uterine leiomyomas

Ex vivo expanded myoma cells or transformed and immortalized by SV40ER lose in vivo tumorigenicity. Injections of uncultured primary myoma cells produce stable xenografts with unique similarity to human fibroids.

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Authors

Michael Drosch, M.Sc., Jörn Bullerdiek, XX, Thomas M. Zollner, M.D., Florian Prinz, M.D., Ph.D., Markus Koch, Ph.D., Nicole Schmidt, Ph.D.

Volume 99, Issue 3, Pages 927-935.e6, 1 March 2013

Abstract

Objective:

To develop a predictive mouse model for uterine fibroids.

Design:

Human fibroid cells xenografted to immunodeficient mice.

Setting:

University and industrial research center.

Animals:

Immunodeficient scid/beige mice.

Interventions:

Subcutaneous and intra-uterine injection of fibroid-derived cells, SV40 transformation of primary cells by lentiviral transduction, proliferation determined by immunohistochemistry, FISH.

Main outcomes:

Characterization of primary and immortalized cells by Western blot and soft agar assay, determination of in vivo tumorigenicity, comparative histology and immunohistochemistry, FISH.

Results:

Tumorigenicity of primary myoma cells disappears upon in vitro culture. Transformation and immortalization does not restore or conserve the in vivo growth potential of cultured cells. Injection of primary cells into myometrium of mice leads to xenografts with a leiomyoma-like histology.

Conclusion:

Primary myoma cells are suited to generate fibroid-like xenografts for studying pathogenesis without genetic modifications. In contrast, in vitro culture abolishes transplantability and neither transformation nor immortalization is sufficient to restore tumorigenic capacity.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)02444-2/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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