Expression of survivin in human oocytes and preimplantation embryos

The study demonstrated that a specific survivin mRNA and protein are expressed in human oocytes and embryos. Embryos secrete survivin into culture media, and survivin concentrations are positively correlated with embryo cleavage rates.

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Authors

Hanna Balakier, Ph.D., Rong Xiao, M.Sc., John Zhao, M.Sc., Shirin Zaver, B.Sc., Ewa Dziak, M.Sc., Katarzyna Szczepanska, Ph.D., Michal Opas, Ph.D., Shang-mian Yie, Ph.D., Clifford Librach, M.D.

Volume 99, Issue 2, Pages 518-525, February 2013

Abstract

Objective:

To determine whether 1) survivin is expressed in human oocytes and embryos; 2) embryos grown in vitro secrete survivin protein; and 3) survivin levels are correlated with embryo cleavage rates.

Design:

Experimental.

Setting:

University-affiliated IVF clinic.

Patient(s):

Couples undergoing IVF-ET cycles.

Intervention(s):

Conventional reverse transcriptase–polymerase chain reaction (PCR), real-time PCR, immunohistochemistry, Western blot on oocytes, embryos and control choriocarcinoma JEG-3 cells, and ELISA analysis of conditioned culture media.

Main Outcome Measure(s):

Detection of survivin mRNA and protein in oocytes and preimplantation embryos, and in JEG-3 cancer cells. Detection of survivin concentrations in embryo culture media.

Result(s):

Survivin mRNA and protein were expressed during human oocyte maturation, from GV to MII stage, and throughout embryo development, from pronuclear stage to blastocyst stage. Survivin was localized predominantly in the cytoplasm of all cells examined, and in oocytes on the chromatin of metaphase chromosomes and midbodies. Western blot analysis of human oocyte and cancer cell extracts detected a full-length (primary) survivin band of 16.5 kDa. Survivin was also detected in conditioned media samples from embryo cultures and showed a positive correlation with embryo cleavage rates.

Conclusion:

Our data has demonstrated, for the first time, that both human oocytes/embryos express and secret survivin, suggesting that survivin may play an important role in human oogenesis and embryogenesis.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)02239-X/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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