Cytokine gene polymorphisms in northern Indian women with recurrent miscarriages

This study highlights the risk involving various genotypes and alleles of the interleukin (IL) 4 (C590T), IL-6 (G174C), IL-10 (1082A/G and 819C/T), and interferon-g (+874 A/T) polymorphism with recurrent miscarriage.

Like Comment

Authors

Farah Parveen, M.Sc., Anju Shukla, M.D, Suraksha Agrawal, M.Sc., Ph.D.

Volume 99, Issue 2, Pages 433-440.e2, February 2013

Abstract

Objective:

To determine association of cytokine gene polymorphism with risk for recurrent miscarriages (RM).

Design:

Retrospective case-control study on northern Indian RM cases versus control subjects.

Setting:

Medical facility.

Patient(s):

A total of 200 women with at least three unexplained spontaneous abortions before 20 weeks of gestation.

Intervention(s):

Subjects were genotyped by the PCR amplification followed by restriction digestion based method and allele-specific oligonucleotides method.

Main Outcome Measure(s):

Detection of pro- and antiinflammatory gene polymorphism genotypes and allele frequencies.

Result(s):

We applied dominant and recessive models of inheritance showing no association among Th2: IL-10(592 C/A) and TGF-β gene polymorphisms, while significant association was observed between Th2: IL-4(C590T), IL-6(G174C), IL-10 (1082A/G & 819 C/T) and Th1: IFN-γ(+874 A/T) with RM when compared to controls. However, when CART (classification and regression tree analysis) was applied this effect disappeared and demonstrated that IL-10 plays an important role in maintenance of pregnancy.

Conclusion(s):

IL-10 act as immunosuppressive by keeping a balance of pro and anti-inflammatory signs that coordinate the satisfactory development of pregnancy, placental growth and remodeling, for favorable pregnancy outcome.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)02244-3/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.