A microfluidic device to reduce treatment time of intracytoplasmic sperm injection

We developed a microfluidic device that can reduce the treatment time of intracytoplasmic sperm injection and compared the treatment time for porcine sperm injection using the method employing this device and the conventional microdroplet method.

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Authors

Koji Matsuura, Ph.D., Takuya Uozumi, M.S., Takuya Furuichi, Ph.D., Ikuyo Sugimoto, M.S., Mieko Kodama, B.S., Hiroaki Funahashi, Ph.D.

Volume 99, Issue 2, Pages 400-407, February 2013

Abstract

Objective:

To develop a microfluidic device that can reduce the intracytoplasmic sperm injection (ICSI) treatment time by increasing sperm concentration.

Design:

We compared the ICSI treatment time required for porcine sperm using a method employing the microfluidic device and one using the conventional microdroplet method.

Settings:

Academic research laboratories at Okayama University.

Animal(s):

Reproductive cells of porcine sperm, oocytes, and embryos.

Intervention(s):

Cell manipulations, ICSI, and embryo culture.

Main Outcome Measure(s):

Average ICSI treatment time and sperm concentration.

Result(s):

The average ICSI treatment time (mean ± SEM) using the method with the microfluidic device for poor-quality semen (sperm concentration, 2.0 × 104 cells/mL) was significantly shorter than the treatment time using the conventional microdroplet method (265 ± 15 seconds [n = 43] vs. 347 ± 19 seconds [n = 50]). When diluted semen with a sperm concentration of 2.0 × 105 cells/mL was used, no significant difference was observed between the two methods (n = 50 and n = 48).

Conclusion(s):

The microfluidic device can reduce the time required for ICSI treatment that is used to increase sperm concentration in poor-quality semen samples. The results suggest that this device may be clinically useful for ICSI treatment in human assisted reproductive technology.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)02322-9/fulltext


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Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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