Progestin induced heart and neural crest derivatives expressed transcript 2 is associated with fibulin 1 expression in human endometrial stromal cells

Progestin-induced heart and neural crest derivatives expressed transcript 2 contributes to fibulin-1 expression in human endometrial stromal cells.

Like Comment

Authors

Hisayuu Cho, M.D., Hidetaka Okada, M.D., Tomoko Tsuzuki, M.D., Akemi Nishigaki, Ph.D., Katsuhiko Yasuda, M.D., Hideharu Kanzaki, M.D.

Volume 99, Issue 1, Pages 248-255.e2, January 2013

Abstract

Objective:

To investigate whether heart and neural crest derivatives expressed transcript 2 (HAND2) regulates fibulin-1 (FBLN1) expression during decidualization of human endometrial stromal cells (ESCs).

Design:

In vitro experiment.

Setting:

Research laboratory.

Patient(s):

Twenty-four patients undergoing hysterectomy for benign reasons.

Intervention(s):

ESCs were cultured with various progestins (medroxyprogesterone acetate [MPA], norethisterone, levonorgestrel, dienogest, and P), E2, dexamethasone, and/or 8-bromoadenosine 3′, 5′-cyclic monophosphate (8-Br-cAMP). HAND2 and FBLN1 were silenced by small interfering RNA technology.

Main Outcome Measure(s):

HAND2 and FBLN1 expression levels were assessed by real-time polymerase chain reaction and Western blot analysis.

Result(s):

MPA or E2 + MPA increased HAND2 mRNA levels in ESCs in a time- and dose-dependent manner, and this stimulatory effect was blocked by RU-486 (P receptor antagonist). HAND2 was increased by E2 + MPA earlier than FBLN1. Simultaneous MPA and 8-Br-cAMP treatment synergistically enhanced HAND2 mRNA levels. P and all the progestins significantly increased HAND2 mRNA levels, whereas E2, 8-Br-cAMP, or dexamethasone alone had no effect. Silencing of HAND2 expression significantly reduced FBLN1 expression, whereas FBLN1 silencing had no effect on HAND2 expression.

Conclusion(s):

These results suggest that progestin-induced HAND2 contributes to FBLN1 expression in human ESCs.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)02175-9/fulltext


Go to the profile of Fertility and Sterility

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

No comments yet.