Development and characterization of an endometrial tissue culture model for study of early implantation events

An improved endometrial tissue culture system was developed. The cultured tissue responded to steroids, allowed leukemia inhibitory factor–dependent blastocyst attachment, and underwent decidualized reaction in vitro.

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Authors

Tian-Min Ye, M.D., Ph.D., Ronald T. K.Pang, Ph.D., Carmen O. N. Leung, M.Med.Sc., Weimin Liu, Ph.D., William S. B.Yeung, Ph.D.

Vol 98, Issue 6, Pages 1581-1589

Abstract

Objective:

To improve and characterize an endometrial tissue culture model.

Design:

Experimental study of the characteristics of mouse endometrial tissue cultured on amniotic membrane matrix.

Setting:

University research laboratory.

Animals:

Sexually mature female ICR mice.

Interventions:

Histological examination of the cultured endometrial tissues. The attachment rates of the cultured tissues to implantation blastocysts under various conditions were determined.

Main Outcome measures:

Morphometric analysis of the cultured tissues. Blastocyst attachment rate and expression of decidualization markers cylcooxygenase-2, connexin 43, and peroxisome proliferator-activated receptor δ.

Results:

Endometrial tissues could be grown on AMM for 3 days with morphometric parameters similar to the in vivo pseudopregnant control. The cultured tissues responded to the surrounding steroid environment. Morphometric assessment indicated medium containing 63.5 nmol/L progesterone and 0.9 nmol/L estradiol provided the best support. The condition allowed attachment of about 60% of the cocultured blastocysts. A small percentage of the attached blastocyst started to penetrate the luminal epithelium within 28 hours. The attachment rate was significantly reduced with prior treatment of the cultured endometrium with anti-leukaemia inhibitory factor antibody. The attached blastocyst induced decidualization around the attachment site.

Conclusions:

The model is useful for the study on implantation in the mouse.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)02065-1/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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Go to the profile of Liubov
Liubov 7 months ago

Dear Tian-Min Ye and Department of Obstetrics and Gynecology team! My name is Liubov. I am a Ph.D. student at Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia. My Thesis is devoted to models for studying implantation of the mouse embryo. I am trying to create my own model for studying implantation of the embryo using primary cultures of mouse endometrial epithelial and stromal cells. 

I was really impressed by your study: "Development and characterization of an endometrial tissue culture model for study of early implantation events". Especially experiments in which you determined physiologic steroid concentrations in mouse serum to adjust the composition of the medium were thorough and incredible.

I am going to treat my model endometrium-like construct with hormonal treatment regimen that you developed. I've found in your article concentrations of the hormones and serum but I can't find final composition of culture medium that you used to culture mouse endometrium. Can you please send me full composition of culture medium? It would help me a lot to improve my model.  If it is possible please contact me by e-mail:  luba.ranaway-94@yandex.ru

Best regards

Liubov